Exploring the clinical application and biological function of novel tsRNA tsRNA-Asp-5–0002 in hepatocellular carcinoma

IF 2.6 3区生物学 Q2 GENETICS & HEREDITY

Gene Pub Date : 2025-03-29 DOI:10.1016/j.gene.2025.149459

Xinyue Ma , Bairong Chen , Yelan Tang , Shaoqing Ju , Rongrong Jing , Wei Feng , Wei Zong

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引用次数: 0

Abstract

Background

Hepatocellular Carcinoma (HCC) is a highly fatal cancer, which is the most common malignant tumor globally. Recently, tRNA-derived small RNA (tsRNA) is a type of non-coding RNA that is closely related to various diseases. However, their precise existence and function in HCC remain unclear. We investigated the role of a novel tsRNA tsRNA-Asp-5–0002 in HCC.

Methods

We screened novel tsRNAs in the TCGA database. There are significant differences in tsRNA-Asp-5–0002 expression in HCC compared with other digestive system tumors. qRT-PCR was applied to confirm the level of tsRNA-Asp-5–0002 in 150 HCC patients, 50 patients with benign liver lesions, and 120 healthy controls. tsRNA-Asp-5–0002 mimics was constructed to transfect HCC cells and explore the effect of tsRNA-Asp-5–0002 on malignant phenotype of HCC. Bioinformatics analysis was used to predict the potential regulatory pathways of tsRNA-Asp-5–0002 in HCC.

Results

(1) tsRNA-Asp-5–0002 is down-regulated in HCC. (2) qRT-PCR detection tsRNA-Asp-5–0002 had high sensitivity and specificity. (3) Low-expressed tsRNA-Asp-5–0002 was related to TNM stage, differentiation, and LNM, and the ROC curve indicated that tsRNA-Asp-5–0002 had a better diagnostic performance for HCC. (4) tsRNA-Asp-5–0002 inhibits the proliferation and migration of HCC through the PLCB1/Wnt axis.

Conclusions

tsRNA-Asp-5–0002 can work as an auxiliary biomarker for HCC diagnosis. Over-expression of tsRNA-Asp-5–0002 restrains the malignant process of HCC, which may offer fresh tactics for the adjuvant treatment of HCC.

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探讨新型tsRNA- asp -5-0002在肝细胞癌中的临床应用及生物学功能。

背景：肝细胞癌（HCC）是一种高致死率的癌症，是全球最常见的恶性肿瘤。trna衍生小RNA （tRNA-derived small RNA, tsRNA）是近年来发现的一类与多种疾病密切相关的非编码RNA。然而，它们在HCC中的确切存在和功能尚不清楚。我们研究了一种新的tsRNA tsRNA- asp -5-0002在HCC中的作用。方法：从TCGA数据库中筛选新的tsRNAs。与其他消化系统肿瘤相比，tsRNA-Asp-5-0002在HCC中的表达有显著差异。应用qRT-PCR技术对150例HCC患者、50例肝脏良性病变患者和120例健康对照进行tsRNA-Asp-5-0002水平的检测。构建tsRNA-Asp-5-0002模拟物转染HCC细胞，探讨tsRNA-Asp-5-0002对HCC恶性表型的影响。利用生物信息学分析预测tsRNA-Asp-5-0002在HCC中的潜在调控途径。结果：(1)tsRNA-Asp-5-0002在HCC中表达下调。(2) qRT-PCR检测tsRNA-Asp-5-0002具有较高的灵敏度和特异性。(3) tsRNA-Asp-5-0002低表达与TNM分期、分化、LNM相关，ROC曲线显示tsRNA-Asp-5-0002对HCC有较好的诊断价值。(4) tsRNA-Asp-5-0002通过PLCB1/Wnt轴抑制HCC的增殖和迁移。结论：tsRNA-Asp-5-0002可作为HCC诊断的辅助生物标志物。tsRNA-Asp-5-0002的过表达抑制了HCC的恶性进程，可能为HCC的辅助治疗提供新的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene 生物-遗传学

CiteScore

6.10

自引率

2.90%

发文量

718

审稿时长

42 days

期刊介绍： Gene publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses.