Monocyte-Platelet Aggregates Are Major Source of BDNF after Bacterial Stimulation of Human Peripheral Blood Immune Cells

IF 4.5 3区 医学 Q2 IMMUNOLOGY
Fabien Sarcletti, Marco Dijmarescu, Michael Eigenschink, Nadja Wukowits, Barbara Oehler, Tanja Mayer, Sarah Pell, Anastasia Tandecki, David Seki, Andreas Spittler, David Berry, Angelika Berger, Lukas Wisgrill
{"title":"Monocyte-Platelet Aggregates Are Major Source of BDNF after Bacterial Stimulation of Human Peripheral Blood Immune Cells","authors":"Fabien Sarcletti,&nbsp;Marco Dijmarescu,&nbsp;Michael Eigenschink,&nbsp;Nadja Wukowits,&nbsp;Barbara Oehler,&nbsp;Tanja Mayer,&nbsp;Sarah Pell,&nbsp;Anastasia Tandecki,&nbsp;David Seki,&nbsp;Andreas Spittler,&nbsp;David Berry,&nbsp;Angelika Berger,&nbsp;Lukas Wisgrill","doi":"10.1002/eji.202451538","DOIUrl":null,"url":null,"abstract":"<p>The gut microbiota and the immune system are closely connected, influencing early-life brain development. Brain-derived neurotrophic factor (BDNF), crucial for neuronal development, has been demonstrated to be produced by certain immune cells. However, the modulation of BDNF during bacterial antigen and metabolite challenge remains elusive. We investigate the effects of bacterial-derived antigens and metabolites on BDNF secretion in human PBMCs. Although BDNF levels were altered during stimulation, a specific cellular origin of BDNF within PBMCs was indeterminate. Positive magnetic separation of monocytes eliminated both the stimulant-induced BDNF secretion and reduced monocyte-platelet aggregates. Conversely, elevated platelet counts significantly increased BDNF levels, indicating that platelets, when interacting with monocytes and exposed to bacterial antigens, are likely the dominant source of BDNF in PBMC cultures. As previously described, platelets are a crucial source of circulating peripheral blood BDNF. Our findings emphasize the importance of the interplay between immune-blood cell complexes during microbial stimulation in regulating BDNF levels. This highlights the necessity of investigating such interactions to better understand the early-life gut-brain axis.</p>","PeriodicalId":165,"journal":{"name":"European Journal of Immunology","volume":"55 4","pages":""},"PeriodicalIF":4.5000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eji.202451538","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/eji.202451538","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The gut microbiota and the immune system are closely connected, influencing early-life brain development. Brain-derived neurotrophic factor (BDNF), crucial for neuronal development, has been demonstrated to be produced by certain immune cells. However, the modulation of BDNF during bacterial antigen and metabolite challenge remains elusive. We investigate the effects of bacterial-derived antigens and metabolites on BDNF secretion in human PBMCs. Although BDNF levels were altered during stimulation, a specific cellular origin of BDNF within PBMCs was indeterminate. Positive magnetic separation of monocytes eliminated both the stimulant-induced BDNF secretion and reduced monocyte-platelet aggregates. Conversely, elevated platelet counts significantly increased BDNF levels, indicating that platelets, when interacting with monocytes and exposed to bacterial antigens, are likely the dominant source of BDNF in PBMC cultures. As previously described, platelets are a crucial source of circulating peripheral blood BDNF. Our findings emphasize the importance of the interplay between immune-blood cell complexes during microbial stimulation in regulating BDNF levels. This highlights the necessity of investigating such interactions to better understand the early-life gut-brain axis.

Abstract Image

单核细胞-血小板聚集体是细菌刺激人外周血免疫细胞后BDNF的主要来源
肠道微生物群和免疫系统密切相关,影响着生命早期的大脑发育。脑源性神经营养因子(BDNF)对神经元发育至关重要,已被证明是由某些免疫细胞产生的。然而,BDNF在细菌抗原和代谢物攻击过程中的调节仍然是难以捉摸的。我们研究了细菌来源的抗原和代谢物对人外周血单核细胞BDNF分泌的影响。虽然BDNF水平在刺激过程中发生改变,但pbmc中BDNF的特定细胞来源尚不确定。单核细胞的正磁分离消除了兴奋剂诱导的BDNF分泌和减少的单核细胞-血小板聚集。相反,升高的血小板计数显著增加BDNF水平,表明血小板与单核细胞相互作用并暴露于细菌抗原时,可能是PBMC培养中BDNF的主要来源。如前所述,血小板是循环外周血BDNF的重要来源。我们的研究结果强调了微生物刺激过程中免疫血细胞复合物在调节BDNF水平中的相互作用的重要性。这凸显了研究这种相互作用以更好地理解早期肠道-大脑轴的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
8.30
自引率
3.70%
发文量
224
审稿时长
2 months
期刊介绍: The European Journal of Immunology (EJI) is an official journal of EFIS. Established in 1971, EJI continues to serve the needs of the global immunology community covering basic, translational and clinical research, ranging from adaptive and innate immunity through to vaccines and immunotherapy, cancer, autoimmunity, allergy and more. Mechanistic insights and thought-provoking immunological findings are of interest, as are studies using the latest omics technologies. We offer fast track review for competitive situations, including recently scooped papers, format free submission, transparent and fair peer review and more as detailed in our policies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信