Transcriptomic and proteomic analyses reveal the diverse components in the venom of a recently described spider species Macrothele washanensis

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2025-03-27 DOI:10.1016/j.cbd.2025.101491

Mengmeng Zhang , Wenzheng Cai , Menghui Yang , Mengying Zhang , Luke R. Tembrock , Zhibin Yang , Heng Liu , Zizhong Yang

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引用次数: 0

Abstract

Spider venom has been shown to possess a variety of pharmacological activities with promising applications in biomedical and agricultural sciences. However, the molecular structure and functions of these toxins have only been revealed for a few species. In this study, we unveiled the venom composition of Macrothele washanensis venom for the first time using transcriptomics, proteomics, and bioinformatic analyses. A total of 147,492,716 Illumina valid reads were obtained from the transcriptome of the venom glands, and 3,010,024 unigenes were assembled to predict 69 putative toxin sequences, which were classified into 25 toxin superfamilies based on cysteine motifs. Crude venom proteomics analysis identified 43 toxin proteins, including 15 peptide toxins and 28 proteases, including the CAP (CRISP/Allergen/PR) protein family, acetylcholinesterase, metalloproteinases, hyaluronidase, and dermonecrotic toxin. In conclusion, the results of the present study revealed the composition of the venom of M. washanensis, and several peptide toxins with potential medical, such work provides the foundation for further research on the molecular functions of these venom components as well as applied studies for the use of such compounds.

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转录组学和蛋白质组学分析揭示了最近描述的一种蜘蛛物种巨蛛毒液的不同成分

蜘蛛毒液已被证明具有多种药理活性，在生物医学和农业科学中具有广阔的应用前景。然而，这些毒素的分子结构和功能仅在少数物种中被揭示。在本研究中，我们首次利用转录组学、蛋白质组学和生物信息学分析揭示了华山巨鳗毒液的组成。从毒腺转录组中共获得147,492,716个Illumina有效reads，并组装了3,010,024个unigenes，以预测69个假定的毒素序列，根据半胱氨酸基序将其分为25个毒素超家族。粗毒液蛋白质组学分析鉴定出43种毒素蛋白，包括15种多肽毒素和28种蛋白酶，包括CAP （CRISP/Allergen/PR）蛋白家族、乙酰胆碱酯酶、金属蛋白酶、透明质酸酶和皮肤坏死毒素。综上所述，本研究结果揭示了山楂毒的组成成分，以及几种具有潜在药用价值的肽毒素，为进一步研究这些毒素成分的分子功能以及这些化合物的应用研究提供了基础。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.