Raman spectroscopy and imaging of protein droplet formation and aggregation

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-04-01 DOI:10.1016/j.sbi.2025.103041

Matthew D. Watson, Jennifer C. Lee

引用次数: 0

Abstract

Raman microscopy offers a unique combination of chemical and spatial resolution with structural sensitivity. This makes it an ideal tool for studies of protein structural changes in heterogenous samples such as protein liquid–liquid phase separation (LLPS) and amyloid formation. These processes are characterized by the spontaneous assembly of proteins to form either microscopic liquid droplets or insoluble filaments stabilized by β-sheets. LLPS and amyloid formation are closely related, with many proteins that undergo LLPS also forming amyloids. This has led to the proposal that development of β-sheets in droplets is an initiating event in toxic amyloid formation. This review surveys recent applications of Raman microscopic methods to studies of LLPS and amyloid formation both in vitro and in cellulo.

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蛋白质液滴形成和聚集的拉曼光谱和成像

拉曼显微镜提供了化学和空间分辨率与结构灵敏度的独特组合。这使得它成为研究异质样品中蛋白质结构变化的理想工具，如蛋白质液-液相分离（LLPS）和淀粉样蛋白形成。这些过程的特点是蛋白质自发组装形成微观液滴或由β片稳定的不溶性细丝。LLPS与淀粉样蛋白的形成密切相关，许多经历LLPS的蛋白质也会形成淀粉样蛋白。这导致了一种建议，即液滴中β片的发展是毒性淀粉样蛋白形成的起始事件。本文综述了拉曼显微方法在体外和纤维素中研究LLPS和淀粉样蛋白形成的最新应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation