The effect of metformin and sea buckthorn (Hippophae rhamnoides L.) in alleviating the symptoms of type 2 diabetes mellitus in the liver of zucker diabetic fatty rats.

IF 2 4区 医学 Q3 PHYSIOLOGY
Journal of Physiology and Pharmacology Pub Date : 2025-02-01 Epub Date: 2025-03-18 DOI:10.26402/jpp.2025.1.07
K Jaszcza, A K Grzegorzewska, A Sachman, A Kalafova, P Massanyi, A Kovacik, R Dupak, M Capcarova
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Abstract

Sea buckthorn (Hippophae rhamnoides L.) has many beneficial health properties. In our previous study we showed suppression of hyperglycaemia, water intake, decreased sorbitol levels in the lens of the eyes after sea buckthorn m(Hippophae rhamnoides L.) treatment in Zucker diabetic fatty rats (animal model of type 2 diabetes mellitus, T2DM) with better antioxidant properties of dried berries. Afterwards, we decided to compare the effects of metformin (150 mg/kg b.wt.) and sea buckthorn dried berries (500 or 1000 mg/kg b.wt.) or leaves infusion per os administration on the liver histology in Zucker diabetic fatty (ZDF) rats. Moreover, following biochemical parameters in the blood serum were determined: alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, cholesterol and triglycerides. The histological analyses revealed the appearance of lipid droplets within the liver tissue of diabetic ZDF rats which were not observed in the non-diabetic control animals. Administration of both metformin and sea buckthorn caused a decrease in the number of lipid droplets in the liver. In ZDF rats, an increase in AST, ALP, bilirubin, cholesterol and triglyceride in blood serum was noticed. In comparison with diabetic animals, metformin and sea buckthorn administration did not change the biochemical parameters but only a tendency to reduce some biochemical parameters was noticed. The obtained results suggest that sea buckthorn dried fruits as well as leaves infusion may have biological potential to be used in prevention and treatment of metabolic diseases. Next studies are needed to analyse the exact mechanism of action, develop and promote sea buckthorn berries as a functional food or natural therapeutical products.

二甲双胍和沙棘对2型糖尿病大鼠肝脏症状的缓解作用。
沙棘(Hippophae rhamnoides L.)具有许多有益健康的特性。在我们之前的研究中,我们发现沙棘m(Hippophae rhamnoides L.)治疗后,Zucker糖尿病脂肪大鼠(2型糖尿病动物模型,T2DM)的高血糖,水分摄入,眼睛晶状体中山梨醇水平降低,而干浆果具有更好的抗氧化性能。随后,我们决定比较二甲双胍(150 mg/kg b.wt)和沙棘干浆果(500或1000 mg/kg b.wt)或叶片输注对Zucker糖尿病脂肪(ZDF)大鼠肝脏组织学的影响。测定血清生化指标:谷丙转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)、胆红素、胆固醇、甘油三酯。组织学分析显示,糖尿病大鼠肝组织内出现脂滴,这在非糖尿病对照动物中没有观察到。服用二甲双胍和沙棘均可减少肝脏中脂滴的数量。ZDF大鼠血清AST、ALP、胆红素、胆固醇、甘油三酯均升高。与糖尿病动物相比,二甲双胍和沙棘均未改变动物的生化指标,但有降低部分生化指标的趋势。上述结果提示,沙棘干果和沙棘叶浸膏在防治代谢性疾病方面具有潜在的生物学潜力。下一步的研究需要分析确切的作用机制,开发和推广沙棘浆果作为功能性食品或天然治疗产品。
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来源期刊
CiteScore
4.00
自引率
22.70%
发文量
0
审稿时长
6-12 weeks
期刊介绍: Journal of Physiology and Pharmacology publishes papers which fall within the range of basic and applied physiology, pathophysiology and pharmacology. The papers should illustrate new physiological or pharmacological mechanisms at the level of the cell membrane, single cells, tissues or organs. Clinical studies, that are of fundamental importance and have a direct bearing on the pathophysiology will also be considered. Letters related to articles published in The Journal with topics of general professional interest are welcome.
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