Damaris Godinez-Vidal, Javier Narváez-Vásquez, Martha L Orozco-Cárdenas
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引用次数: 0
Abstract
Lettuce (Lactuca sativa L.) plays a pivotal role in global agriculture and food supply chains as a widely cultivated commercial crop. This chapter presents a detailed protocol for transforming lettuce using Agrobacterium tumefaciens. The procedure includes a two-day pre-culture period of cotyledon explants, followed by a 20-min inoculation with Agrobacterium without explant wounding, and a two-day co-culture on the same medium. Afterward, the selection and regeneration phases under kanamycin selection led to the establishment of transgenic plants in the greenhouse. The entire process from the initial explant infection to the greenhouse cultivation of transgenic plants spans roughly 120 days, achieving an average transformation efficiency of 45%. This protocol offers a reliable method for the genetic modification of lettuce, for use in research or crop improvement.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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