Tubule-Specific Compensatory Responses to Cpt1a Deletion in Aged Mice.

Abstract

Background: Fatty acid oxidation (FAO) is the preferred energy pathway in the proximal tubule (PT), and carnitine palmitoyltransferase 1A (CPT1A) is the rate-limiting enzyme of mitochondrial FAO. CPT1A expression and FAO decrease after renal injury. Our recent work demonstrated that genetic deletion of tubular Cpt1a did not significantly worsen the response to injury or aging and did not completely block FAO, suggesting compensatory metabolic pathways1. Additionally, CPT1A was most highly expressed in distal convoluted tubules (DCT), a segment not known for FAO. Therefore, we used single nuclear RNA sequencing to explore a cell-specific responses to aging with high fat diet (HFD-aging), to define compensatory metabolic pathways in PT segments lacking Cpt1a, and to determine the role of Cpt1a in the DCT.

Methods: Cpt1a floxed (Cpt1afl/fl) and tubule-specific conditional Cpt1a knockout (Cpt1aCKO) mice were aged for 2 years with HFD. Single nuclear RNA-sequencing was performed on these HFD-aged mice plus young controls.

Results: HFD-aged mice had increased fibrosis, inflammation, and more injured PT cells than young mice. Whereas PT segments from HFD-aged mice had significant transcriptional changes in metabolism-related pathways, the DCT had more changes in inflammation-related pathways. Compared with floxed mice, HFD-aged Cpt1aCKO mice had increased lipid deposition and increased inflammation but no significant differences in fibrosis or renal function. PT segments from HFD-aged Cpt1aCKO mice had significantly upregulated Hmgcs2, a promoter of ketogenesis and fatty acid oxidation, and upregulated genes in peroxisomal fatty acid oxidation and omega-fatty acid oxidation (CYP4A family) pathways. DCT from HFD-aged Cpt1aCKO mice had decreased expression of DCT-specific markers of cell differentiation.

Conclusions: The upregulated Hmgcs2, peroxisomal fatty acid oxidation genes, and CYP4A genes may compensate for impaired mitochondrial metabolism of long chain fatty acids in PT cells lacking Cpt1a. Our data suggest that CPT1A may be important in maintenance of cell differentiation for DCT.