The First Complete Mitochondrial Genomes for the Genus Dianema (Siluriformes: Callichthyidae): Dianema longibarbis and D. urostriatum.

IF 2.8 3区生物学 Q2 GENETICS & HEREDITY

Genes Pub Date : 2025-03-20 DOI:10.3390/genes16030355

Seong Duk Do, Jae-Sung Rhee

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引用次数: 0

Abstract

Background/objectives: To date, no information is available on the complete mitochondrial genome of the genus Dianema (Siluriformes: Callichthyidae), a callichthyid catfish. In this study, we report on two complete mitochondrial genome sequences of Dianema longibarbis Cope, 1872, and Dianema urostriatum Miranda Ribeiro, 1912, the only two recognized species within the genus Dianema.

Methods: DNA sequencing was performed using the HiSeq platform to obtain their complete mitogenomes. To confirm phylogenetic distance, two phylogenetic trees were established using maximum-likelihood and Bayesian inference methods with all concatenated protein-coding sequences (PCGs) and two ribosomal RNA (rRNA) genes from the D. longibarbis and D. urostriatum mitogenomes, along with 32 mitogenomes retrieved from Siluriformes.

Results: The complete mitogenomes of D. longibarbis and D. urostriatum are 16,493 and 16,495 base pairs in length, respectively. Their nucleotide compositions are 31.79% A, 27.53% T, 25.86% C, and 14.82% G for D. longibarbis, and 31.69% A, 27.04% T, 26.36% C, and 14.91% G for D. urostriatum. Both mitogenomes contain 13 PCGs, 22 transfer RNA (tRNA) genes, and two rRNA genes. Phylogenetic results based on all PCGs and two rRNAs genes confirm D. longibarbis as a sister species to D. urostriatum in the subfamily Callichthyinae.

Conclusions: In contrast to the extensive mitochondrial studies on species in the Corydoradinae, species in the Callichthyinae have been largely understudied. This study provides valuable insights into genetic diversity and evolutionary complexity by presenting the first mitochondrial genome analysis of two Dianema species, a genus within the Callichthyinae.

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滇蝇属（蛭形目：鳞蝇科）的第一个完整线粒体基因组：长尾滇蝇和尿纹状体滇蝇。

背景/目的：到目前为止，还没有关于鳞目鲶鱼Dianema属（Siluriformes: Callichthyidae）完整线粒体基因组的信息。在这项研究中，我们报告了两个完整的线粒体基因组序列的diema longibarbis Cope， 1872年，和diema urostriatum Miranda Ribeiro， 1912年，唯一的两个公认的diema属物种。方法：使用HiSeq平台进行DNA测序，获得完整的有丝分裂基因组。确认系统的距离,建立了两个系统发育树使用最大似然和贝叶斯推理方法与所有连接蛋白质编码序列(pcg)和两个核糖体RNA (rRNA)基因d . longibarbis和d urostriatum mitogenomes,连同32 mitogenomes检索鲇形目。结果：长尾鼠和尾纹鼠的全有丝分裂基因组长度分别为16493和16495个碱基对。它们的核苷酸组成分别为：长刺田鼠的31.79% A、27.53% T、25.86% C和14.82% G，尾纹田鼠的31.69% A、27.04% T、26.36% C和14.91% G。两个有丝分裂基因组包含13个PCGs， 22个tRNA基因和2个rRNA基因。基于所有PCGs和两个rnas基因的系统发育结果证实，长尾纹蝶是鱼亚科尿纹蝶的姐妹种。结论：相对于对紫堇科物种线粒体的广泛研究，糙鳞鱼科物种的研究在很大程度上是不足的。本研究通过首次对两种石鳞鱼（Callichthyinae）的线粒体基因组进行分析，为遗传多样性和进化复杂性提供了有价值的见解。

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来源期刊

Genes GENETICS & HEREDITY-

CiteScore

5.20

自引率

5.70%

发文量

1975

审稿时长

22.94 days

期刊介绍： Genes (ISSN 2073-4425) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to genes, genetics and genomics. It publishes reviews, research articles, communications and technical notes. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.