Cross-Kingdom Communication via Plant-Derived Extracellular Vesicle Nucleic Acids in Genetically Engineered Nicotiana tabacum.

IF 2.8 3区 生物学 Q2 GENETICS & HEREDITY
Genes Pub Date : 2025-03-20 DOI:10.3390/genes16030356
Lorena Urbanelli, Federica Delo, Giada Cerrotti, Emidio Albertini, Jacopo Lucci, Sandra Buratta, Eleonora Calzoni, Stefano Giovagnoli, Luana Lugini, Cristina Federici, Federica Fratini, Valentino Mercati, Carla Emiliani
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引用次数: 0

Abstract

Background/objectives: Plants release extracellularly lipid bilayer-enclosed vesicles of nanometric size that can be retrieved in their fluids. Plant-derived extracellular vesicles (PDEVs) have mostly been involved in modulating host-pathogen interaction, making them a tool for cross-kingdom communication with a key role in plant immunity. In addition, PDEVs have demonstrated promising therapeutic features, not only in terms of intrinsic nutraceutical properties but also of active molecules' delivery. Transgenic plants have been developed for a variety of purposes, i.e., to improve their functional properties like crops, but also to produce therapeutic molecules. However, it is unclear whether transgenes can end up in PDEVs, thus making them a vehicle for their cross-kingdom diffusion into the environment.

Methods: Here, we investigated the association of transgenic DNA and RNA with PDEVs secreted by tobacco (Nicotiana tabacum) engineered to express the neomycine phosphotransferase II (Npt-II) gene. PDEVs were isolated from leaf apoplastic fluid by ultracentrifugation and characterized for their morphology and size. The association of DNA and RNA was assessed by qRT-PCR and their immunomodulatory properties by assaying PDEVs-induced IL1β and IL10 on THP1 monocytes.

Results: Npt-II RNA, but not DNA, could be amplified from PDEVs, whereas no differences were observed between wt and transgenic tobacco PDEVs in terms of immunomodulatory properties.

Conclusions: Although a different behaviour by other types of RNAs or DNAs could still be possible, our findings indicate that in this model, PDEVs are not associated with transgenic DNA, but they can protect RNA, including transgenic RNA, from degradation, contributing to their cross-kingdom spreading.

烟草基因工程中植物源性细胞外囊泡核酸的跨界通讯。
背景/目的:植物释放细胞外脂质双层封闭的纳米大小的囊泡,可以从它们的液体中提取。植物源性细胞外囊泡(PDEVs)主要参与调节宿主与病原体的相互作用,使其成为跨界交流的工具,在植物免疫中起关键作用。此外,PDEVs不仅具有内在的营养特性,而且具有活性分子的传递能力,因此具有良好的治疗特性。转基因植物已被开发用于各种目的,例如,像作物一样改善其功能特性,但也生产治疗分子。然而,目前尚不清楚转基因是否最终会出现在pdev中,从而使它们成为跨界扩散到环境中的载体。方法:研究转基因DNA和RNA与烟草(Nicotiana tabacum)分泌的表达新霉素磷酸转移酶II (Npt-II)基因的PDEVs的关联。利用超离心技术从叶片胞外液中分离到PDEVs,并对其形态和大小进行了表征。qRT-PCR检测DNA和RNA的相关性,并通过检测pdevs诱导的THP1单核细胞上的il - 1β和il - 10来检测它们的免疫调节特性。结果:从烟草PDEVs中可以扩增到Npt-II RNA,但不能扩增到DNA,而wt和转基因烟草PDEVs在免疫调节特性方面没有差异。结论:尽管其他类型的RNA或DNA的不同行为仍然可能存在,但我们的研究结果表明,在该模型中,PDEVs与转基因DNA无关,但它们可以保护RNA(包括转基因RNA)免受降解,从而促进其跨界传播。
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来源期刊
Genes
Genes GENETICS & HEREDITY-
CiteScore
5.20
自引率
5.70%
发文量
1975
审稿时长
22.94 days
期刊介绍: Genes (ISSN 2073-4425) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to genes, genetics and genomics. It publishes reviews, research articles, communications and technical notes. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.
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