3D reconstruction of neuronal allometry and neuromuscular projections in asexual planarians using expansion tiling light sheet microscopy.

IF 6.4 1区 生物学 Q1 BIOLOGY
eLife Pub Date : 2025-03-28 DOI:10.7554/eLife.101103
Jing Lu, Hao Xu, Dongyue Wang, Yanlu Chen, Takeshi Inoue, Liang Gao, Kai Lei
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引用次数: 0

Abstract

The intricate coordination of the neural network in planarian growth and regeneration has remained largely unrevealed, partly due to the challenges of imaging the CNS in three dimensions (3D) with high resolution and within a reasonable timeframe. To address this gap in systematic imaging of the CNS in planarians, we adopted high-resolution, nanoscale imaging by combining tissue expansion and tiling light-sheet microscopy, achieving up to fourfold linear expansion. Using an automatic 3D cell segmentation pipeline, we quantitatively profiled neurons and muscle fibers at the single-cell level in over 400 wild-type planarians during homeostasis and regeneration. We validated previous observations of neuronal cell number changes and muscle fiber distribution. We found that the increase in neuron cell number tends to lag behind the rapid expansion of somatic cells during the later phase of homeostasis. By imaging the planarian with up to 120 nm resolution, we also observed distinct muscle distribution patterns at the anterior and posterior poles. Furthermore, we investigated the effects of β-catenin-1 RNAi on muscle fiber distribution at the posterior pole, consistent with changes in anterior-posterior polarity. The glial cells were observed to be close in contact with dorsal-ventral muscle fibers. Finally, we observed disruptions in neural-muscular networks in inr-1 RNAi planarians. These findings provide insights into the detailed structure and potential functions of the neural-muscular system in planarians and highlight the accessibility of our imaging tool in unraveling the biological functions underlying their diverse phenotypes and behaviors.

无性涡虫神经异速生长和神经肌肉投影的三维重建。
神经网络在涡虫生长和再生中的复杂协调在很大程度上仍未被揭示,部分原因是在合理的时间框架内以高分辨率三维(3D)成像CNS面临挑战。为了解决涡虫中枢神经系统成像的这一空白,我们采用了高分辨率的纳米级成像,结合组织扩张和平铺光片显微镜,实现了高达四倍的线性扩张。利用自动3D细胞分割管道,我们定量分析了400多种野生涡虫在体内平衡和再生过程中的单细胞水平的神经元和肌肉纤维。我们验证了之前观察到的神经元细胞数量变化和肌纤维分布。我们发现,在体内平衡的后期,神经元细胞数量的增加往往滞后于体细胞的快速扩张。通过对涡虫进行高达120纳米分辨率的成像,我们还观察到前后两极明显的肌肉分布模式。此外,我们还研究了β-catenin-1 RNAi对后极肌纤维分布的影响,该影响与前后极性的变化一致。神经胶质细胞与背腹肌纤维紧密接触。最后,我们观察到inr-1 RNAi涡虫的神经-肌肉网络被破坏。这些发现提供了对涡虫神经肌肉系统的详细结构和潜在功能的见解,并强调了我们的成像工具在揭示其不同表型和行为背后的生物学功能方面的可及性。
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来源期刊
eLife
eLife BIOLOGY-
CiteScore
12.90
自引率
3.90%
发文量
3122
审稿时长
17 weeks
期刊介绍: eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.
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