Chemical modification and immobilization of cellulase for simultaneous pretreatment and saccharification of lignocellulosic biomass in ternary deep eutectic solvent

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-23 DOI:10.1016/j.enzmictec.2025.110638

Yue Zhang , Chi Lei , Liangzhi Li , Bin Zou , Tao Xu , Lishi Yan

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引用次数: 0

Abstract

In this study, chemical modification coupled with immobilization of cellulase was developed for simultaneous pretreatment and saccharification (SPS) of lignocellulosic biomass in ternary deep eutectic solvent (DES). Cellulase was firstly modified with acid anhydrides to form citraconic anhydride modified cellulase (CA-Ce) and phthalic anhydride modified cellulase (PA-Ce), respectively. Then the modified cellulases were immobilized on amino-functionalized mesoporous silica nanoparticles (MSN-NH₂) to fabricate CA-Ce@MSN-NH₂ and PA-Ce@MSN-NH₂. A series of physiochemical characterizations were employed to characterize modified cellulases or immobilization carriers. Thermodynamic analysis and deactivation kinetics in ternary DES (NMMO/Bet/OA) revealed that CA-Ce@MSN-NH₂ had desired thermal stability and solvent tolerance, which maintained 80.5 % initial activity at 70 °C and 77.8 % initial activity in 50 % NMMO/Bet/OA. The SPS of bagasse in NMMO/Bet/OA via CA-Ce@MSN-NH₂ resulted in 84.1 % total reducing sugar (TRS) yield at optimal condition.

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木质纤维素生物质在三元深共熔溶剂中同时预处理和糖化的纤维素酶的化学改性和固定化

本研究采用纤维素酶固定化与化学改性相结合的方法，在三元深共熔溶剂（DES）中对木质纤维素生物质进行预处理和糖化（SPS）。首先用酸酐对纤维素酶进行修饰，分别形成柠檬酸酐修饰纤维素酶（CA-Ce）和邻苯二酸酐修饰纤维素酶（PA-Ce）。将改性后的纤维素酶固定在氨基功能化的介孔二氧化硅纳米颗粒（MSN-NH2）上制备CA-Ce@MSN-NH2和PA-Ce@MSN-NH2。采用一系列的物理化学表征来表征改性的纤维素酶或固定化载体。热力学分析和失活动力学表明，三元DES (NMMO/Bet/OA) CA-Ce@MSN-NH2具有良好的热稳定性和耐溶剂性，在70 °C时保持80.5 %的初始活性，在50 %的NMMO/Bet/OA时保持77.8 %的初始活性。通过CA-Ce@MSN-NH2对甘蔗渣在NMMO/Bet/OA中进行SPS处理，最佳条件下总还原糖（TRS）得率为84.1 %。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.