Chemical modification and immobilization of cellulase for simultaneous pretreatment and saccharification of lignocellulosic biomass in ternary deep eutectic solvent

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-23 DOI:10.1016/j.enzmictec.2025.110638

Yue Zhang , Chi Lei , Liangzhi Li , Bin Zou , Tao Xu , Lishi Yan

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引用次数: 0

Abstract

In this study, chemical modification coupled with immobilization of cellulase was developed for simultaneous pretreatment and saccharification (SPS) of lignocellulosic biomass in ternary deep eutectic solvent (DES). Cellulase was firstly modified with acid anhydrides to form citraconic anhydride modified cellulase (CA-Ce) and phthalic anhydride modified cellulase (PA-Ce), respectively. Then the modified cellulases were immobilized on amino-functionalized mesoporous silica nanoparticles (MSN-NH₂) to fabricate CA-Ce@MSN-NH₂ and PA-Ce@MSN-NH₂. A series of physiochemical characterizations were employed to characterize modified cellulases or immobilization carriers. Thermodynamic analysis and deactivation kinetics in ternary DES (NMMO/Bet/OA) revealed that CA-Ce@MSN-NH₂ had desired thermal stability and solvent tolerance, which maintained 80.5 % initial activity at 70 °C and 77.8 % initial activity in 50 % NMMO/Bet/OA. The SPS of bagasse in NMMO/Bet/OA via CA-Ce@MSN-NH₂ resulted in 84.1 % total reducing sugar (TRS) yield at optimal condition.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.