Simultaneous production of linear α-olefins and 2,5-furandicarboxylic acid by combining two recombinant enzymes OleT-ELP and HMFO-ELP

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-22 DOI:10.1016/j.enzmictec.2025.110637

Yaqi Fu , Siyu Mao , Tianyue Liao , Wei Feng

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引用次数: 0

Abstract

The enzyme OleT can utilize H₂O₂ as the co-substrate, and this biocatalysis is an H₂O₂-driven enzymatic catalysis. In this work, OleT was recombinated by being fused to an elastin-like polypeptide (ELP). The recombinant enzyme OleT-ELP exhibits higher stability and resistance to H₂O₂ interference compared to native OleT. OleT-ELP showed improved catalytic efficiency in producing α-olefins via fatty acid decarboxylation. The recombinant 5-hydroxymethylfurfural oxidase (HMFO-ELP) catalyzes the oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA), generating H₂O₂ as a byproduct. Combining OleT-ELP with HMFO-ELP enabled simultaneous conversion of fatty acids and HMF. The in situ H₂O₂ generated by HMFO-ELP was transferred to OleT-ELP, enhancing catalytic efficiencies for both α-olefins and FDCA production.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.