PROGESTERONE TREATMENT POSITIVE EFFECTS IN THE FUNCTION AND INFLAMMATION OF INTESTINE COMPROMISED BYAORTIC OCCLUSION.

IF 2.7 3区医学 Q2 CRITICAL CARE MEDICINE

SHOCK Pub Date : 2025-03-27 DOI:10.1097/SHK.0000000000002595

Brunella Valbão Flora Agostinho, Pedro Luiz Zonta de Freitas, Ivana Ramires Fraga, Lucas Ferreira da Anunciação, Fernanda Yamamoto Ricardo-da-Silva, Ana Cristina Breithaupt-Faloppa, Cristiano Jesus Correia, Luiz Felipe Pinho Moreira

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Abstract

Methods: Male Wistar rats were randomized to 4 groups: Sham, surgically manipulated; IR, animals subjected to I/R; P4 Pre, animals treated with P4 30 minutes before I/R and P4 Post, treated immediately after flow reestablishment. I/R was induced by catheter 2F insufflation in the descending aorta, occluded for 30 min, followed by reperfusion for 2 h. Intestinal function, inflammatory and apoptosis markers, and mesenteric microcirculation were analyzed.

Results: Aortic occlusion led to systemic changes and intestinal homeostasis disruption. The I/R decreased the gastrointestinal transit (Sham 69%, IR 46%; P = 0.0014) and the progesterone reduced impact induced by I/R (IR 46%, P4 Post 60%; P = 0.0461). The treatment prevented the increase of epithelial mucosal barrier permeability and edema formation triggered by I/R (Sham 0.2770 ng/mL vs I/R 5.455 ng/mL; P = 0.0048) and (P4 Pre 2.027 ng/mL; P = 0.0393). It also reduced inflammatory cell infiltration to intestinal tissue (IR 0.5876 vs P4 Pre 0.1818; P = 0.0003 and P4 Post 0.1393 stained area/tissue area; P < 0.0001), modulating the release of inflammatory mediators and apoptosis markers (IR 0.1806 vs P4 Pre 0.0468; P = 0.0452).

Conclusion: Progesterone treatment was effective in modulating the neutrophil response and other inflammatory markers, decreased apoptosis, and improved gastrointestinal function triggered by I/R.

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黄体酮治疗对主动脉阻塞损害肠功能和炎症的积极影响。

方法：雄性Wistar大鼠随机分为4组：假手术组；IR，受I/R约束的动物；P4 Pre，在I/R前30分钟给予P4； P4 Post，在血流恢复后立即给予。在降主动脉内注入2F导管诱导I/R，闭塞30 min，再灌注2 h。分析肠功能、炎症和凋亡指标、肠系膜微循环。结果：主动脉阻塞导致全身改变和肠道内平衡破坏。I/R减少胃肠道运输(Sham 69%, IR 46%；P = 0.0014)，孕酮降低I/R诱导的影响(IR 46%, Post 60%；P = 0.0461)。治疗可阻止I/R引起的上皮粘膜屏障通透性增加和水肿形成(Sham 0.2770 ng/mL vs I/R 5.455 ng/mL；P = 0.0048)和(P4 Pre: 2.027 ng/mL；P = 0.0393)。它还能减少炎症细胞对肠组织的浸润(IR 0.5876 vs P4 Pre 0.1818；P = 0.0003和P4后染色面积/组织面积0.1393；P < 0.0001)，调节炎症介质和凋亡标志物的释放(IR 0.1806 vs P4 Pre 0.0468；P = 0.0452)。结论：黄体酮治疗可有效调节中性粒细胞反应和其他炎症标志物，减少细胞凋亡，改善I/R引起的胃肠道功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

SHOCK 医学-外科

CiteScore

6.20

自引率

3.20%

发文量

199

审稿时长

1 months

期刊介绍： SHOCK®: Injury, Inflammation, and Sepsis: Laboratory and Clinical Approaches includes studies of novel therapeutic approaches, such as immunomodulation, gene therapy, nutrition, and others. The mission of the Journal is to foster and promote multidisciplinary studies, both experimental and clinical in nature, that critically examine the etiology, mechanisms and novel therapeutics of shock-related pathophysiological conditions. Its purpose is to excel as a vehicle for timely publication in the areas of basic and clinical studies of shock, trauma, sepsis, inflammation, ischemia, and related pathobiological states, with particular emphasis on the biologic mechanisms that determine the response to such injury. Making such information available will ultimately facilitate improved care of the traumatized or septic individual.