The Chinese gut virus catalogue reveals gut virome diversity and disease-related viral signatures.

IF 10.4 1区 生物学 Q1 GENETICS & HEREDITY
Qiulong Yan, Liansha Huang, Shenghui Li, Yue Zhang, Ruochun Guo, Pan Zhang, Zhixin Lei, Qingbo Lv, Fang Chen, Zhiming Li, Jinxin Meng, Jing Li, Guangyang Wang, Changming Chen, Hayan Ullah, Lin Cheng, Shao Fan, Wei You, Yan Zhang, Jie Ma, Shanshan Sha, Wen Sun
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引用次数: 0

Abstract

Background: The gut viral community has been increasingly recognized for its role in human physiology and health; however, our understanding of its genetic makeup, functional potential, and disease associations remains incomplete.

Methods: In this study, we collected 11,286 bulk or viral metagenomes from fecal samples across large-scale Chinese populations to establish a Chinese Gut Virus Catalogue (cnGVC) using a de novo virus identification approach. We then examined the diversity and compositional patterns of the gut virome in relation to common diseases by analyzing 6311 bulk metagenomes representing 28 disease or unhealthy states.

Results: The cnGVC contains 93,462 nonredundant viral genomes, with over 70% of these being novel viruses not included in existing gut viral databases. This resource enabled us to characterize the functional diversity and specificity of the gut virome. Using cnGVC, we profiled the gut virome in large-scale populations, assessed sex- and age-related variations, and identified 4238 universal viral signatures of diseases. A random forest classifier based on these signatures achieved high accuracy in distinguishing diseased individuals from controls (AUC = 0.698) and high-risk patients from controls (AUC = 0.761), and its predictive ability was also validated in external cohorts.

Conclusions: Our resources and findings significantly expand the current understanding of the human gut virome and provide a comprehensive view of the associations between gut viruses and common diseases. This will pave the way for novel strategies in the treatment and prevention of these diseases.

中国肠道病毒目录揭示了肠道病毒的多样性和疾病相关的病毒特征。
背景:肠道病毒群落在人体生理和健康中的作用日益得到认可;然而,我们对其基因组成、功能潜力和疾病关联的理解仍然不完整。方法:在本研究中,我们从中国大规模人群的粪便样本中收集了11,286个散装或病毒宏基因组,采用从头鉴定方法建立了中国肠道病毒目录(cnGVC)。然后,我们通过分析代表28种疾病或不健康状态的6311个大块宏基因组,研究了与常见疾病相关的肠道病毒组的多样性和组成模式。结果:cnGVC包含93,462个非冗余病毒基因组,其中70%以上是现有肠道病毒数据库中未包含的新病毒。这一资源使我们能够表征肠道病毒的功能多样性和特异性。使用cnGVC,我们在大规模人群中分析了肠道病毒组,评估了性别和年龄相关的变异,并确定了4238种疾病的通用病毒特征。基于这些特征的随机森林分类器在区分患病个体与对照组(AUC = 0.698)和高危患者与对照组(AUC = 0.761)方面取得了很高的准确率,其预测能力也在外部队列中得到了验证。结论:我们的资源和发现极大地扩展了目前对人类肠道病毒组的理解,并提供了肠道病毒与常见疾病之间关系的全面观点。这将为治疗和预防这些疾病的新策略铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genome Medicine
Genome Medicine GENETICS & HEREDITY-
CiteScore
20.80
自引率
0.80%
发文量
128
审稿时长
6-12 weeks
期刊介绍: Genome Medicine is an open access journal that publishes outstanding research applying genetics, genomics, and multi-omics to understand, diagnose, and treat disease. Bridging basic science and clinical research, it covers areas such as cancer genomics, immuno-oncology, immunogenomics, infectious disease, microbiome, neurogenomics, systems medicine, clinical genomics, gene therapies, precision medicine, and clinical trials. The journal publishes original research, methods, software, and reviews to serve authors and promote broad interest and importance in the field.
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