A Novel Molecular Regulatory Network in Bone Marrow Mesenchymal Stem Cells for Age-Related Osteoporosis.

IF 3 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Clinical Endocrinology Pub Date : 2025-03-27 DOI:10.1111/cen.15239

Ming-Dong Gao, Xiao-Jun Wang, Peng-Biao Li, Qian-Qian Dong, Li-Min Tian

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引用次数: 0

Abstract

Background: This study evaluates the miRNA-mRNA regulatory networks that potentially influence the senescence mechanisms of bone marrow mesenchymal stem cells (BMSCs) in age-related osteoporosis (ARO). By identifying these networks, the study aims to offer new molecular markers and therapeutic targets for ARO.

Methods: Five mRNA datasets were analyzed to identify common differentially expressed genes associated with senescence and osteoporosis. Seven hub genes were found to be enriched in the PI3K-Akt signaling pathway, and 22 hub miRNAs potentially regulating these genes. Primary BMSCs were harvested and cultured from seven younger, non-osteoporotic individuals and six older adults with osteoporosis. Expression levels of the hub genes and miRNAs were validated using quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Expression analysis showed that integrin subunit beta 3 (ITGB3), receptor tyrosine kinase ligand (KITLG), platelet-derived growth factor (PDGFB), and their associated regulatory miRNAs, exhibited significant differences between the two BMSC groups.

Conclusion: A newly identified miRNA-mRNA regulatory network may mediate ARO via the PI3K-Akt signaling pathway in BMSCs. These molecular insights provide a foundation for potential therapeutic interventions targeting age-related osteoporosis.

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一种新的骨髓间充质干细胞与年龄相关性骨质疏松症的分子调控网络。

背景：本研究评估了在年龄相关性骨质疏松症（ARO）中可能影响骨髓间充质干细胞（BMSCs）衰老机制的miRNA-mRNA调控网络。通过识别这些网络，该研究旨在为ARO提供新的分子标记和治疗靶点。方法：对5个mRNA数据集进行分析，找出与衰老和骨质疏松相关的常见差异表达基因。7个枢纽基因在PI3K-Akt信号通路中富集，22个枢纽mirna可能调控这些基因。从7名年轻的非骨质疏松个体和6名患有骨质疏松症的老年人中收获并培养原代骨髓间充质干细胞。利用实时定量聚合酶链式反应（qRT-PCR）验证枢纽基因和mirna的表达水平。结果：表达分析显示，整合素亚单位β 3 （ITGB3）、受体酪氨酸激酶配体（KITLG）、血小板衍生生长因子（PDGFB）及其相关的调控mirna在两组BMSC中表现出显著差异。结论：在骨髓间质干细胞中，新发现的miRNA-mRNA调控网络可能通过PI3K-Akt信号通路介导ARO。这些分子见解为针对年龄相关性骨质疏松症的潜在治疗干预提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Endocrinology 医学-内分泌学与代谢

CiteScore

6.40

自引率

3.10%

发文量

192

审稿时长

1 months

期刊介绍： Clinical Endocrinology publishes papers and reviews which focus on the clinical aspects of endocrinology, including the clinical application of molecular endocrinology. It does not publish papers relating directly to diabetes care and clinical management. It features reviews, original papers, commentaries, correspondence and Clinical Questions. Clinical Endocrinology is essential reading not only for those engaged in endocrinological research but also for those involved primarily in clinical practice.