Steroid-protein adduct in the lens of chicken embryo following application of prednisolone esters.

Abstract

Protein--prednisolone adducts were detected by semiquantitative radioimmunoassay in both water- and urea soluble fractions of homogenates from lenses of chicken embryos, given systemically single doses (100 microliters each) of four prednisolone esters. The findings were correlated with macroscopic changes of lens transparency. The highest concentrations of protein-associated immunoreactive material were found after prednisolone sulphate application, associated with the most apparent loss of transparency.