Urinary total superoxide dismutase activity is low in rheumatoid patients, and its low levels are associated with higher DAS28 scores

IF 7.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Free Radical Biology and Medicine Pub Date : 2025-03-25 DOI:10.1016/j.freeradbiomed.2025.03.035

Ahmet Kor , Merve Yalçın , Göktuğ Okyar , Tahire Betül Kor , İsmail Doğan , Ebru Atalar , Hatice Ecem Konak , Pınar Akyüz Dağlı , Dudu Çelik Tam , Sema Işık , Bünyamin Polat , Salim Neşelioğlu , Özcal Erel , Şükran Erten

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Abstract

Aim

Rheumatoid factor and anti-citrullinated peptide antibodies are generally used to diagnose rheumatoid arthritis (RA). However, these antibodies are negative in 15–20 % of RA cases. This study aimed to investigate new biomarkers that can be used in diagnosing RA and evaluating disease activity.

Methods

This study included 99 RA patients and 94 healthy volunteers. Modified Disease Activity Score28 (DAS28) erythrocyte sedimentation rate (ESR) was used for disease activity score. Thiol/disulfide molecules were measured using an automatic spectrophotometric method, and ischemia-modified albumin was measured using an albumin-cobalt binding test. A spectrophotometer calculated urinary and serum total superoxide dismutase (SOD) activities at 420 nm wavelength.

Results

In the RA group, serum IMA level was higher (unstandardized beta [B]: 5.552, Odds Ratio [OR]: 250.1, CI 95 %: 1.29–48219.1, p: .040), and urinary total SOD activities were significantly lower (B: 2.640 OR: .071, CI 95 %: .027–.192, p < 0.0001) than in the control group. A cut-off value of 5.06 for urinary total SOD was found to have a sensitivity of 97.9 % and a specificity of 97 % in distinguishing RA patients from healthy controls (AUC [95 %Cl] = .986 [.967–1.000], p < 0.001). Urinary total SOD activity was significantly lower in the RA subgroup with high disease activity compared to the groups with moderate disease activity (B: .954, OR: 2.596, CI 95 %: 1.104–6.103, p: .029) and low disease activity (B: 1.251, OR: 3.494, CI 95 %: 1.143–10.678, p: .028).

Conclusion

This study showed that urinary total SOD activity has high sensitivity and specificity in distinguishing RA patients from healthy controls and that RA patients with higher DAS28 ESR activity scores have lower urinary total SOD activity.

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类风湿患者尿总超氧化物歧化酶活性低，其低水平与较高的DAS28评分相关

目的类风湿性关节炎（RA）的诊断常用类风湿因子和抗瓜氨酸肽抗体。然而，这些抗体在15 - 20%的类风湿性关节炎病例中呈阴性。本研究旨在探索可用于RA诊断和评估疾病活动性的新生物标志物。方法纳入99例RA患者和94名健康志愿者。疾病活动性评分采用改良疾病活动性评分28 （DAS28）红细胞沉降率（ESR）。硫醇/二硫分子用自动分光光度法测定，缺血修饰白蛋白用白蛋白-钴结合试验测定。分光光度计计算尿和血清总超氧化物歧化酶（SOD）在420 nm波长下的活性。结果RA组血清IMA水平较高（未标准化β [B]: 5.552，比值比[OR]: 250.1, CI 95%: 1.29-48219.1, p: 0.040），尿总SOD活性显著降低（B: 2.640 OR: 0.071, CI 95%: 0.027 -）。192, p <；0.0001)高于对照组。尿总超氧化物歧化酶（SOD）的临界值为5.06，区分RA患者与健康对照的敏感性为97.9%，特异性为97% (AUC [95% Cl] = .986 [.967-1.000], p <；0.001)。与中度疾病活动度组（B: 0.954, OR: 2.596, CI 95%: 1.104-6.103, p: 0.029）和低疾病活动度组（B: 1.251, OR: 3.494, CI 95%: 1.143-10.678, p: 0.028）相比，疾病活动度高的RA亚组尿总SOD活性显著降低。结论尿总SOD活性在区分RA患者与健康对照具有较高的敏感性和特异性，DAS28 ESR活性评分较高的RA患者尿总SOD活性较低。

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来源期刊

Free Radical Biology and Medicine 医学-内分泌学与代谢

CiteScore

14.00

自引率

4.10%

发文量

850

审稿时长

22 days

期刊介绍： Free Radical Biology and Medicine is a leading journal in the field of redox biology, which is the study of the role of reactive oxygen species (ROS) and other oxidizing agents in biological systems. The journal serves as a premier forum for publishing innovative and groundbreaking research that explores the redox biology of health and disease, covering a wide range of topics and disciplines. Free Radical Biology and Medicine also commissions Special Issues that highlight recent advances in both basic and clinical research, with a particular emphasis on the mechanisms underlying altered metabolism and redox signaling. These Special Issues aim to provide a focused platform for the latest research in the field, fostering collaboration and knowledge exchange among researchers and clinicians.