IgG antibody response to Mycobacterium tuberculosis curli pili (MTP) in people from different geographical regions in Sub-Saharan Africa

IF 2.8 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2025-03-20 DOI:10.1016/j.tube.2025.102634

Koobashnee Pillay , Theresa Coetzer , Catherine Connolly , Balakrishna Pillay , Thamsanqa Chiliza , Kogieleum Naidoo , Jayne Sutherland , Thumbi Ndung'u , Harriet Mayanja-Kizza , Manormoney Pillay

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引用次数: 0

Abstract

Previously, a slot blot or an indirect enzyme-linked immunosorbent assay (ELISA) using a synthetic or purified MTP antigen, conceptually demonstrated IgG antibody induction in pulmonary TB patients, albeit with small sample sizes and differing sensitivity. Therefore, we evaluated an IgG MTP ELISA in larger populations from The Gambia (n = 549), Uganda (n = 161), and South Africa (n = 193), comprising human immunodeficiency virus (HIV) positive and negative, with microbiologically confirmed active TB. The association between the IgG level and demographic characteristics was determined by multivariate logistic regression. The sensitivity (44.8–61.2 %) and specificity (33.4–78.5 %) varied in the three cohorts. Anti-MTP antibody titres differed between the TB positive and negative groups within the South African and The Gambian cohorts (p < 0.001), but not in Uganda (p = 0.35). Antibodies were detected in HIV positive and negative patients and were reduced at 6-month follow-up after treatment (p > 0.067). The study verified previous findings that anti-MTP antibodies, and therefore MTP antigen, are produced during active TB. However, the accuracy of the MTP-IgG ELISA was low, and is therefore not suitable as a target product profile in the high burden TB areas investigated. Further studies are needed to clarify the variable reactivities in different geographical areas.

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撒哈拉以南非洲不同地理区域人群对毛卷曲结核分枝杆菌（MTP）的IgG抗体反应

以前，使用合成或纯化的MTP抗原的槽印迹或间接酶联免疫吸附试验（ELISA）在概念上证明了IgG抗体对肺结核患者的诱导作用，尽管样本量小且敏感性不同。因此，我们在冈比亚（n = 549）、乌干达（n = 161）和南非（n = 193）的更大人群中评估了IgG MTP ELISA，包括人类免疫缺陷病毒（HIV）阳性和阴性，微生物学证实为活动性结核病。IgG水平与人口统计学特征之间的关系通过多变量logistic回归确定。三个队列的敏感性（44.8 - 61.2%）和特异性（33.4 - 78.5%）各不相同。抗mtp抗体滴度在南非和冈比亚队列中结核阳性和阴性组之间存在差异(p <；0.001)，但乌干达没有（p = 0.35）。在HIV阳性和阴性患者中检测到抗体，并在治疗后6个月随访时降低(p >；0.067)。该研究证实了以前的发现，即在活动性结核病期间产生抗MTP抗体，从而产生MTP抗原。然而，MTP-IgG ELISA的准确性较低，因此不适合作为所调查的高负担结核病地区的目标产品。需要进一步的研究来阐明不同地理区域的不同反应性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.