Yixuan Ming, Tianyi Zhou, Bin Lu, Yemaiza Ojeda-Lassalle, Pasquale Valerio, Lu Wang, Mi Jin
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引用次数: 0
Abstract
Triton X-100 is one of the most widely used detergents for cell lysis in gene therapy product manufacturing. However, due to the aquatic toxicity of the degradation product, Triton X-100 is regulated by the European Chemical Agency as a substance of very high concern. In this study, we aim to identify eco-friendly detergent alternatives to replace Triton X-100. Tween 20, Tween 80, n-Dodecyl-β-D-maltoside (DDM), and Triton CG-110 were tested and compared with Triton X-100. Triton CG-110 demonstrated similar performance during the harvest and subsequent purification process for multiple AAV vectors at different scales. Therefore, Triton CG-110 represents an effective and environmentally safe detergent alternative to Triton X-100.
期刊介绍:
Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries.
Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.