Alcalase immobilization in iota-carrageenan-matrix hydrogel beads derived from the macroalga Solieria filiformis

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-22 DOI:10.1016/j.enzmictec.2025.110636

Alan Portal D'Almeida , Luciana Rocha Barros Gonçalves , Tiago Lima de Albuqueque da Silva , Roberto Fernandez-Lafuente , Ivanildo José da Silva Jr.

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引用次数: 0

Abstract

This study aims to immobilize Bacillus licheniformis (Alcalase) protease in iota-carrageenan (ιCAR) matrix hydrogels via adsorption. CAR was extracted from macroalgae Solieria filiformis and used to produce hydrogels using Al^3 + as the gelling agent. Subsequently, enzyme immobilization was performed at 25ºC, for 120 min using particles of ∼2.0 mm diameter, varying the medium pH values (7.0, 8.0, and 9.0). The immobilization at pH 8.0 resulted in the biocatalyst with the highest immobilization yield (100 %), expressed activity (88.9 %), and mass activity (10.4 U/g) for 1.0 mg/g of enzyme loading. When using particles with different diameters (1.0, 2.0, and 3.0 mm), the best results were obtained using 1.0 mm particles. This permitted a 100 % immobilization yield, 95.8 % expressed activity, and high mass activity (11.2 U/g). The lyophilized biocatalyst presented varying macro-pore diameters, ranging from 21 to 126 µm. The immobilized biocatalyst was 11 times more stable than the soluble enzyme at 60ºC and pH 8.0 and presented > 80 % retained activity in the pH range 6.0–9.0.

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将钙化酶固定在由大型藻类丝状藻（Solieria filiformis）提取的藻胶基质水凝胶珠中

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.