LINC01088 Targets miR-195-5p to Promote Proliferation and Migration and Reduce Apoptosis in the Inhibition of Carotid Artery Stenosis.

IF 2.3 4区 医学 Q2 HEMATOLOGY
Huoquan Tang, Shuo Sun, Yali Zhang, Ying Jin, Caijiao Wang, Chunchun Xu, Yanfeng Zhang, Li Chen, Defeng Wu
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Abstract

Carotid artery stenosis (CAS) often goes undetected until it reaches an advanced stage, which can result in serious complications. The present study evaluated the potential of long noncoding RNA (lncRNA) LINC01088 as a biomarker for CAS. 92 CAS patients and 92 healthy controls (Control group) were included. RT-qPCR was performed to assess the relative levels of LINC01088 and miR-195-5p. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic potential of LINC01088. The relationship between LINC01088 and miR-195-5p was identified by luciferase reporter assay. Proliferation, migration, and apoptosis in human aortic endothelial cells (HAECs) were assessed using CCK8, transwell, and flow cytometry assay. DAVID was employed for Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses. CAS patients showed decreased LINC01088 expression and increased miR-195-5p expression compared to Control, with a negative correlation between their expression levels in CAS. LINC01088 demonstrated high sensitivity and specificity in distinguishing CAS patients from healthy individuals. LINC01088 directly targets miR-195-5p. Upregulation of LINC01088 reversed the effects of ox-LDL treatment, restoring proliferation and migration while reducing apoptosis in HAECs. However, miR-195-5p mimic reduced the protection of LINC01088 on HAECs proliferation, migration, and apoptosis. For miR-195-5p target genes, GO revealed protein metabolism pathways and KEGG highlighted the p53 and MAPK signaling pathways. The present study revealed the diagnosis value of LINC01088. LINC01088 reversed ox-LDL-induced proliferation, apoptosis, and migration by acting as sponges of miR-195-5p in HAECs. LINC01088 may serve as a protective biomarker in CAS progression.

LINC01088靶向miR-195-5p促进颈动脉狭窄增殖迁移,减少细胞凋亡。
颈动脉狭窄(CAS)通常直到发展到晚期才被发现,这可能导致严重的并发症。本研究评估了长链非编码RNA (lncRNA) LINC01088作为CAS生物标志物的潜力。纳入92例CAS患者和92例健康对照(对照组)。采用RT-qPCR方法评估LINC01088和miR-195-5p的相对水平。采用受试者工作特征(ROC)曲线评价LINC01088的诊断潜力。LINC01088和miR-195-5p之间的关系通过荧光素酶报告基因检测确定。采用CCK8、transwell和流式细胞术检测人主动脉内皮细胞(HAECs)的增殖、迁移和凋亡。DAVID受雇于京都基因与基因组百科全书(KEGG)和基因本体(GO)分析。与对照组相比,CAS患者的LINC01088表达降低,miR-195-5p表达升高,两者在CAS中的表达水平呈负相关。LINC01088在区分CAS患者和健康人方面具有很高的敏感性和特异性。LINC01088直接靶向miR-195-5p。LINC01088的上调逆转了ox-LDL处理的作用,恢复了haec的增殖和迁移,同时减少了凋亡。然而,miR-195-5p模拟物降低了LINC01088对haec增殖、迁移和凋亡的保护作用。对于miR-195-5p靶基因,GO揭示了蛋白质代谢途径,KEGG突出了p53和MAPK信号通路。本研究揭示了LINC01088的诊断价值。LINC01088通过在haec中充当miR-195-5p的海绵,逆转ox- ldl诱导的增殖、凋亡和迁移。LINC01088可作为CAS进展中的保护性生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.40
自引率
3.40%
发文量
150
审稿时长
2 months
期刊介绍: CATH is a peer-reviewed bi-monthly journal that addresses the practical clinical and laboratory issues involved in managing bleeding and clotting disorders, especially those related to thrombosis, hemostasis, and vascular disorders. CATH covers clinical trials, studies on etiology, pathophysiology, diagnosis and treatment of thrombohemorrhagic disorders.
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