Genome-wide identification and analysis of GH1-containing H1 histones among poplar species.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Ping Li, Jing Wang, Qimin Zhang, Anmin Yu, Rui Sun, Aizhong Liu
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引用次数: 0

Abstract

Histone H1s are basic nuclear proteins, which played key role in the binding of DNA and nucleosome, eventually the stability of eukaryotic chromatin. In most species, H1s possess an evolutionarily conserved nucleosome-DNA binding globular domain (GH1), which is conserved between species, especially in mammals. However, there is limited information on the phylogeny, structure and function of H1s in poplar. In the present research, 21 GH1-containing proteins found in Populus trichocarpa were classified into three subgroups (H1s, Myb (SANK) GH1 and AT-hook GH1) based on their domains. The Populus H1 proteins contained lysine-rich N-, C-terminal tails and a conserved GH1 domain, particularly the characteristic amino acids in the helix and strand structures of the five H1 subtypes. The phylogenetic and structure diversity analysis of GH1 proteins across different Populus species and model plants revealed three conserved subgroups with characteristic amino acids. The variation in the number of members across the five subtypes was consistent with the evolutionary relationships among Populus species. The conserved characteristic amino acids among same Populus subtype can be served as markers for subtype identification. Furthermore, the abundance analysis of H1s in Populus indicated their unique functions in young tissues and stages, which may be related to DNA methylation. The consistent expression pattern of H1 across Populus species was in accordance with collinearity pairs. Present analyses provided valuable information on the diversity and evolution of H1s in Populus, advocating further research of H1s in plants.

杨树含gh1 H1组蛋白的全基因组鉴定与分析。
组蛋白H1s是一种基本的核蛋白,在DNA与核小体的结合,最终决定真核染色质的稳定性中起着关键作用。在大多数物种中,H1s具有进化上保守的核小体- dna结合球形结构域(GH1),这种结构域在物种之间是保守的,特别是在哺乳动物中。然而,目前对杨树H1s基因的系统发育、结构和功能的研究还很有限。本研究将毛杨中21种含GH1的蛋白根据结构域划分为3个亚群(H1s、Myb (sunk) GH1和AT-hook GH1)。杨树H1蛋白含有富含赖氨酸的N端、c端和一个保守的GH1结构域,特别是5种H1亚型的螺旋和链结构中的特征氨基酸。通过对不同杨种和模式植物GH1蛋白的系统发育和结构多样性分析,揭示了3个具有特征氨基酸的保守亚群。5个亚型间成员数量的差异与杨树种间的进化关系一致。同一杨树亚型间保守的特征氨基酸可作为亚型鉴定的标记。此外,对杨树中H1s基因的丰度分析表明,它们在幼龄组织和发育阶段具有独特的功能,这可能与DNA甲基化有关。H1在杨树种间的一致表达模式符合共线性对。本研究为揭示杨树H1s基因的多样性及其进化过程提供了有价值的信息,为进一步开展植物H1s基因的研究奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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