Exploring the Antibiofilm and Antibacterial Potential of Datura stramonium and Prosopis farcta Against Gram-Positive and Gram-Negative Bacteria

IF 2.1 4区 医学 Q3 PHARMACOLOGY & PHARMACY
Soheila Shahroodian, Maryam Mirshekar, Maryam Koupaei, Shiva Mirkalantari, Nour Amirmozafari
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Abstract

Background: The rise of drug-resistant bacteria poses a significant challenge to global healthcare, underscoring the urgent need for new therapeutic interventions. In this study, ethanolic extracts of Datura stramonium and Prosopis farcta, sourced from Northern Iran, were examined for their antibacterial properties against Staphylococcus aureus, Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa.

Methods: The antibacterial activities of the medicinal extracts were examined in this study using disc diffusion, microbroth dilution, and agar well diffusion methods against both Gram-positive and Gram-negative microorganisms. In addition, the extracts were assessed for toxicity using the MTT assay on HT29 cells. The biofilm-inhibitory effects of the extracts were also investigated.

Results: The results showed that the ethanolic extracts from D. stramonium and P. farcta have strong antibacterial activity against S. aureus. However, their activity against P. aeruginosa and A. baumannii is less pronounced. The extracts showed significant antibiofilm capabilities against the tested bacteria at both MIC and 2× MIC concentrations (p < 0.05). The MTT test showed HT29 cells were more sensitive to P. farcta extract than D. stramonium, especially at 200 μg/mL concentration.

Conclusion: The findings demonstrate that ethanolic extracts of P. farcta and D. stramonium exhibit significant antibacterial activity, particularly against S. aureus, while also effectively disrupting bacterial biofilms. These results suggest that these extracts possess considerable potential as alternative therapeutic agents against drug-resistant bacterial infections. Further investigation is warranted to elucidate the specific mechanisms of action and to explore their efficacy in clinical applications.

Abstract Image

探索曼陀罗和法罗贝对革兰氏阳性和革兰氏阴性细菌的抗菌膜和抑菌潜力
背景:耐药细菌的增加对全球医疗保健构成了重大挑战,强调了对新的治疗干预措施的迫切需要。在这项研究中,研究了产自伊朗北部的曼陀罗(Datura stramonium)和假葡萄球菌(Prosopis farcta)的乙醇提取物对金黄色葡萄球菌、大肠杆菌、鲍曼不动杆菌、肺炎克雷伯菌和铜绿假单胞菌的抗菌性能。方法:采用圆盘扩散法、微肉汤稀释法和琼脂孔扩散法对革兰氏阳性菌和革兰氏阴性菌进行抑菌试验。此外,采用MTT法评估提取物对HT29细胞的毒性。并对其生物膜抑制作用进行了研究。结果:结果表明,曲曲霉和法尔塔的乙醇提取物对金黄色葡萄球菌具有较强的抗菌活性。然而,它们对铜绿假单胞菌和鲍曼假单胞菌的活性不太明显。在MIC和2倍MIC浓度下,提取物对被测细菌均显示出显著的抗菌膜能力(p <;0.05)。MTT试验显示,HT29细胞对棘球龙提取物的敏感性高于棘球龙提取物,特别是在200 μg/mL浓度下。结论:该研究结果表明,P. farcta和D. stramonium的乙醇提取物具有显著的抗菌活性,特别是对金黄色葡萄球菌,同时也能有效地破坏细菌的生物膜。这些结果表明,这些提取物具有相当大的潜力,作为替代治疗药物耐药细菌感染。需要进一步的研究来阐明其具体的作用机制,并探讨其临床应用的有效性。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
226
审稿时长
6 months
期刊介绍: The Journal of Clinical Pharmacy and Therapeutics provides a forum for clinicians, pharmacists and pharmacologists to explore and report on issues of common interest. Reports and commentaries on current issues in medical and pharmaceutical practice are encouraged. Papers on evidence-based clinical practice and multidisciplinary collaborative work are particularly welcome. Regular sections in the journal include: editorials, commentaries, reviews (including systematic overviews and meta-analyses), original research and reports, and book reviews. Its scope embraces all aspects of clinical drug development and therapeutics, including: Rational therapeutics Evidence-based practice Safety, cost-effectiveness and clinical efficacy of drugs Drug interactions Clinical impact of drug formulations Pharmacogenetics Personalised, stratified and translational medicine Clinical pharmacokinetics.
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