Simultaneous separation of oils, proteins and polysaccharides from soybean: Microstructure, rheological properties and oxidative stability studies of emulsions

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-03-19 DOI:10.1016/j.molliq.2025.127418

Zhe Huang, Kaiwen Xing, Jiaye Yu, Yaqing Chen, Tong Wang, Fei Wu, Dianyu Yu

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Abstract

In this study, the enzyme pretreatment ultrasound-assisted three-phase separation (E/U-TPP) method was used to achieve the simultaneous extraction and separation of soybean oil (SBO), protein (SBP), and polysaccharide (SBPS) by varying the ultrasonic power, and to prepare oil-in-water (O/W) emulsions. The SBO, SBP, SBPS and emulsions were characterized and determined. The findings indicated that E/U_120W-TPP increased the extraction of SBO, SBP, and SBPS by more than 20 % compared to TPP. Under E/U_120W-TPP conditions, the SBO was found to contain 22.77 % saturated fatty acids and 74.30 % unsaturated fatty acids. The particle size of SBP reached a minimum of 203 nm indicating that sonication can lead to the unfolding of proteins, while the highest surface hydrophobicity of 11,045 likewise verified this claim. After the determination of monosaccharides, it was found that SBPS is mainly composed of galactose, arabinose and xylose, which are heteropolysaccharides. The chemical structure and surface morphology of SBPS were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM), and the results showed that E/U_120W-TPP did not disrupt the structure of SBPS, while SEM showed that SBPS has a finely fragmented structure. In order to better state the emulsion, E/U_120W-TPP extracted the optimal SBP, which was then mixed with the optimal SBPS and subjected to sonication. This process resulted in an emulsion with the smallest particle size of 125 nm, which was achieved under the treatment of 120 W. Optical microscopy and laser confocal microscopy also verified this conclusion. The rheological characterization demonstrated that the viscosity of the system exhibited a tendency to decrease and then increase with the application of increasing ultrasonic power. The lowest viscosity value of the emulsion was observed in the E/U_120W-120 state. Additionally, the rheological properties of G’’ were observed to be greater than G’’ with increasing frequency, indicating that the emulsion exhibited elastic behavior. These results provide a theoretical basis for the sustainable utilization of SBO, SBP and SBPS from soybeans, and the functional properties of the emulsions were investigated, which can help to rationally select the optimal processing conditions and construct a stable emulsion system.

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.