Aqueous extract of Ormocarpum cochinchinense augments proliferation and promotes osteogenesis in osteoblast like MG63 cells

IF 1.5 Q3 ORTHOPEDICS

Journal of orthopaedics Pub Date : 2025-03-21 DOI:10.1016/j.jor.2025.03.035

Preethi Vincent, Krishna Priya Mani

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引用次数: 0

Abstract

Background

Bone tissue engineering requires a high restorative index as a demand from modern day requirements. Therapeutic agents derived from natural sources play a vital role as modulators in promoting bone regeneration. Ormocarpum cochinchinense, a shrub, is used by the traditional healers of southern East Asia to treat bone fractures. However, there remains a dearth in the exploration of its bone healing properties.

Methods

The present study reports a systematic analysis of the aqueous extract of Ormocarpum cochinchinense (OCE) as a repository of natural bio-actives capable of eliciting in vitro biomineralization and thereby osteogenesis. MTT assay, and Live/dead cell staining assay were used to evaluate the cell viability of MG-63 cells. Alizarin staining evaluated the mineralization by OCE, and ALP enzyme activity was measured both qualitatively and quantitatively. Gene expression of osteogenic markers (Runx2, ALP, Collagen type 1, BMP2, Osteocalcin, and Osteopontin) was measured by RT-qPCR.

Results

The MTT, and Live/Dead staining results exemplified significant proliferation index. Augmented biomineralization as early as 7 days was validated by Alizarin Red staining with increased mineralized nodules. The activity of Alkaline phosphatase (an early bone marker) was significantly enhanced by OCE. Furthermore, the expression levels of early and late osteogenic markers were increased profoundly after OCE treatment in a dose-dependent manner.

Conclusion

The present study demonstrates the potential of Ormocarpum cochinchinense to promote osteogenesis, exemplifying its potential for bone tissue engineering.

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来源期刊

Journal of orthopaedics ORTHOPEDICS-

CiteScore

3.50

自引率

6.70%

发文量

202

审稿时长

56 days

期刊介绍： Journal of Orthopaedics aims to be a leading journal in orthopaedics and contribute towards the improvement of quality of orthopedic health care. The journal publishes original research work and review articles related to different aspects of orthopaedics including Arthroplasty, Arthroscopy, Sports Medicine, Trauma, Spine and Spinal deformities, Pediatric orthopaedics, limb reconstruction procedures, hand surgery, and orthopaedic oncology. It also publishes articles on continuing education, health-related information, case reports and letters to the editor. It is requested to note that the journal has an international readership and all submissions should be aimed at specifying something about the setting in which the work was conducted. Authors must also provide any specific reasons for the research and also provide an elaborate description of the results.