Mediator-29 limits Caenorhabditis elegans fecundity.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY

Genetics Pub Date : 2025-05-08 DOI:10.1093/genetics/iyaf051

Qi Fan, Christopher Tran, Wei Cao, Roger Pocock

引用次数: 0

Abstract

Mediator is an evolutionarily conserved multiprotein complex that acts as a critical coregulator of RNA polymerase II-mediated transcription. While core Mediator components are broadly required for transcription, others govern specific regulatory modules and signaling pathways. Here, we investigated the function of MDT-29/MED29 in the Caenorhabditis elegans germ line. We found that endogenously tagged MDT-29 is ubiquitously expressed and concentrated in discrete foci within germ cell nuclei. Functionally, depleting MDT-29 in the germ line during larval development boosted fecundity. We determined that the increase in progeny production was likely caused by a combination of an expanded germline stem cell pool and decreased germ cell apoptosis. Thus, MDT-29 may act to optimize specific gene expression programs to control distinct germ cell behaviors, providing flexibility to progeny production in certain environments.

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中介-29限制秀丽隐杆线虫繁殖力。

中介体是一种进化上保守的多蛋白复合物，作为RNA聚合酶ii介导的转录的关键协同调节剂。虽然转录广泛需要核心中介成分，但其他成分控制特定的调节模块和信号传导途径。在此，我们研究了MDT-29/MED29在秀丽隐杆线虫种系中的功能。我们发现内源性标记的MDT-29在生殖细胞核内的离散灶中普遍表达和集中。从功能上讲，在幼虫发育过程中消耗生殖系中的MDT-29提高了繁殖力。我们确定后代产量的增加可能是由生殖系干细胞池的扩大和生殖细胞凋亡的减少共同引起的。因此，MDT-29可能会优化特定的基因表达程序，以控制不同的生殖细胞行为，为某些环境下的后代生产提供灵活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.