Rapid and Sensitive Glutathione Detection Using Water-Soluble Fluorescent Bimetallic Nanoparticles

IF 2.6 4区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
ChemNanoMat Pub Date : 2025-02-28 DOI:10.1002/cnma.202400642
Aditi Mahanty, Amit Akhuli, Chinmayee Patra, Joyoti Ghosh, Moloy Sarkar
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Abstract

This work aims to design and develop a simple but effective strategy for the selective and sensitive detection of glutathione (GSH) in aqueous medium by exploiting fluorescent bimetallic nanoparticles. To achieve this, water-soluble, fluorescent silver-capped gold nanoparticles (F−AgAu) has been synthesized and characterized through conventional methods. The sensing behaviour of the F−AgAu for several analytes of interest has been investigated by employing steady state and time-resolved spectroscopic techniques. Signaling strategy has been conceptualized by exploiting both “turn-on” and “turn-off” condition of the fluorescent nanoparticles against specific analytes in sequential manner. The method is based on the F−AgAu/Hg2+ system, where the initial fluorescence from F−AgAu is quenched (“turn-off”) by Hg2+. Time-resolved fluorescence studies have revealed that a photoinduced electron transfer (PET) process from nanoparticle to Hg2+ is primarily responsible for the fluorescence quenching behavior. Interestingly, in the presence of GSH, the fluorescence of the nanoparticle is found to be recovered (“on” state). The fluorescence “on” state of the nanoparticles is attributed to the competitive affinity of Hg2+ for thesurface ligand, GSH. More interestingly, it has been demonstrated that the present signaling strategy is quite effective in detecting GSH in various fruits and food samples at low concentration levels.

Abstract Image

水溶性荧光双金属纳米颗粒快速灵敏检测谷胱甘肽
本工作旨在设计和开发一种简单而有效的方法,利用荧光双金属纳米颗粒选择性和灵敏地检测水介质中的谷胱甘肽(GSH)。为了实现这一目标,我们合成了水溶性的荧光银包覆金纳米粒子(F−AgAu),并通过常规方法对其进行了表征。利用稳态和时间分辨光谱技术研究了F−AgAu对几种感兴趣的分析物的传感行为。通过利用荧光纳米颗粒对特定分析物的“开启”和“关闭”条件,信号策略已被概念化。该方法基于F−AgAu/Hg2+体系,其中F−AgAu的初始荧光被Hg2+淬灭(“关闭”)。时间分辨荧光研究表明,从纳米颗粒到Hg2+的光诱导电子转移(PET)过程是荧光猝灭行为的主要原因。有趣的是,在谷胱甘肽的存在下,纳米颗粒的荧光被发现恢复(“开启”状态)。纳米颗粒的荧光“开启”状态归因于Hg2+对表面配体GSH的竞争亲和力。更有趣的是,已经证明,目前的信号策略是相当有效的检测谷胱甘肽在各种水果和食品样品中的低浓度水平。
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来源期刊
ChemNanoMat
ChemNanoMat Energy-Energy Engineering and Power Technology
CiteScore
6.10
自引率
2.60%
发文量
236
期刊介绍: ChemNanoMat is a new journal published in close cooperation with the teams of Angewandte Chemie and Advanced Materials, and is the new sister journal to Chemistry—An Asian Journal.
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