Bioprospecting of Novel Dye-Tolerant Actinomycetes for the Fabric Dye degradation and Its Phytotoxicity Analysis

Abstract

The current study aims to isolate novel dye-tolerant actinomycetes from dye-contaminated soil. The antibacterial efficiency of the selected isolates (VITVR4 and VITVR6) displays the highest inhibitory action against Pseudomonas aeruginosa (22 mm) and considerable antibacterial activity against all other species. Both the isolates exhibit γ-hemolysis, causing no pathogenicity in hemolytic activity. The goal of the current work is the removal of fabric azo dyes, including methyl orange (MO), Reactive blue-19, and Rhodamine B, by the actinomycetes strains from dye-contaminated soil. The higher percentage of decolorization of MO, Reactive blue-19, and Rhodamine B by isolate VITVR4 is observed as 86%, 79%, and 74%, respectively. While the isolate VITVR6 exhibits the decolorization percentage of the azo dyes MO, Reactive blue-19, and Rhodamine B as 72%, 80%, and 88%, respectively. The isolated strains were identified as Microbacterium endophyticum (GenBank: OQ625846) and Brevibacterium casei (GenBank: OQ625847) according to the phenotypic and genotypic methods. Furthermore, phytotoxicity analysis of microbially treated dye products indicated less toxicity in Vigna radiata seeds. The findings could potentially serve as a useful tool for efficiently decolorizing dyes released from textile effluent by actinomycetes species.