Comprehensive investigation of antibacterial, cytotoxic, and antioxidant activities of Ipomoea staphylina flower extract with isolation and characterization of Beta-sitosterol

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2025-03-13 DOI:10.1016/j.molstruc.2025.142045

Lakshmanan Narayanan , T. Maruthavanan , R. Vaithiyanathan , Geetha Venkatesan , K.E. Vivekanandan , Gayathri Kaliyannan , Paskalis Sahaya Murphin Kumar , Mary Sahaya Anisha John Bosco , Govindhasamy Murugadoss , S.R. Suseem

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Abstract

This study aims to bridge the gap by scientifically investigating the ethnopharmacological evidence surrounding the I. staphylina plant. In this research we evaluated the antibacterial and cytotoxic abilities of I. staphylina flower extracts. The extract with the highest antioxidant activity underwent cytotoxicity testing against HCT-116 cells. Further analysis confirmed the presence of Beta-sitosterol through HPTLC, followed by its isolation via column chromatography. Antibacterial tests showed significant activity against various gram-positive and gram-negative bacteria, with notable resistance against S. aureus, P. mirabilis, and P. aeruginosa. The IPFPE extract exhibited the highest inhibition against S. aureus (16 mm) and P. mirabilis (15 mm), while ethanolic extract showed potent activity against S. aureus and P. aeruginosa (16 mm, 15 mm). The anticancer study, using ethanolic and pet-ether extracts on HCT-116 colon cancer cells and assessed via the MTT assay, revealed significant reductions in cell viability due to apoptosis induced by secondary metabolites (Beta-sitosterol) and antioxidants. A significant reduction in cell viability was observed, with IC₅₀ values of 51.44 µM (IPFE) and 52.74 µM (IPFPE), a 50 % growth inhibition was observed, highlighting vigorous pro-apoptotic activity. These results underscore the potential of I. staphylina flower extracts as promising antibacterial and chemotherapeutic agents, warranting further research to validate their efficacy.

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.