Characterization of a novel short-chain dehydrogenase from Sugiyamaella lignohabitans and its application in the degradation of patulin

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-20 DOI:10.1016/j.enzmictec.2025.110630

Wei Xu , Jiayi Yao , Chenyu Song , Ting Xue , Wanmeng Mu

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Abstract

Patulin (PAT) is a widespread and hazardous fungal toxin that is commonly found in fruits and grain crops, threatening global food safety. Increasing attention has been focused on biological approaches for PAT decontamination in recent years. This study successfully identified a short-chain dehydrogenase with PAT-degrading activity derived from Sugiyamaella lignohabitans and made a preliminary characterization of the recombinant enzyme Suli-SDR. The Suli-SDR displayed the optimal activity at pH 7.0 and 80 °C, indicating a good activity when applied in the pasteurization procedure of the apple juice. Suli-SDR with a final concentration of 1 μg/mL could degrade 88 % of PAT (50 μg/mL) within 12 h, revealing its superior degradation performance. After measuring the parameters related to the quality of apple juice, including vitamin C, titratable acids, total phenols, etc., it was found that there was no significant effect on the parameters of apple juice after enzymatic treatment by Suli-SDR. In a short summary, this study evaluated the catalytic ability of Suli-SDR and explored the feasibility of enzyme on PAT control in practical production.

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.