Integrated analysis of lipid metabolism and differentially expressed genes reveal seed oil accumulation in field muskmelon

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-03-19 DOI:10.1016/j.ygeno.2025.111031

Jiyuan Wang , Hengyi Ren , Jie Zhu , Yahui Li , Jie Liu , Hu Li , Chun Liu , Yupeng Fan , Huijun Zhang

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引用次数: 0

Abstract

Field muskmelon (Cucumis melo L. var. agrestis Naud.), a novel oil crop, contains a high amount of lipids in seeds. However, the high-resolution profiles and dynamic regulation of its lipids remain largely unknown. This study identified the lipids and analyzed their dynamic changes using UHPLC-MS/MS. We identified 2533 lipid molecules in the seeds, including 7 categories and 47 sub-classes, with the higher proportions of glycerolipids (41.02 %) and glycerophospholipids (28.11 %). Moreover, the content of glycerolipids was the highest, particularly for triacylglycerol lipid molecules. Additionally, the expression patterns of differentially expressed genes (DEGs) showed a close correlation with lipid accumulation, especially within the plant hormone signaling pathway. Notably, the sufficient supply of 18:1-CoA, coupled with a high expression level of CmFAD2, contributed significantly to the high linoleic acid (68.56 %) content in field muskmelon seeds. Our findings offer insights that could enhance the comprehensive understanding of lipids in field muskmelon, and facilitate the breeding of field muskmelon.

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脂质代谢和差异表达基因的综合分析揭示了甜瓜籽粒油脂积累。

甜瓜（Cucumis melo L. var. agrestis Naud.）是一种新型油料作物，其种子中含有大量的脂质。然而，其脂质的高分辨率剖面和动态调节在很大程度上仍然未知。本研究利用UHPLC-MS/MS对脂质进行鉴定，并分析其动态变化。共鉴定出脂类分子2533个，包括7类47亚类，其中甘油脂类（41.02 %）和甘油磷脂类（28.11 %）的比例较高。此外，甘油脂的含量最高，特别是三酰基甘油脂类分子。此外，差异表达基因（DEGs）的表达模式与脂质积累密切相关，特别是在植物激素信号通路中。值得注意的是，供应充足的18:1-CoA，加上CmFAD2的高表达水平，是田间甜瓜种子中亚油酸含量高（68.56 %）的重要原因。本研究为进一步全面了解甜瓜的脂质特性，促进甜瓜的选育提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.