Mesencephalic astrocyte-derived neurotrophic factor upregulates CHOP and ATF6 in the rat retina with retinitis pigmentosa.

IF 1.2 4区医学 Q4 GENETICS & HEREDITY

Ophthalmic Genetics Pub Date : 2025-03-20 DOI:10.1080/13816810.2025.2482610

Zhenya Gao, Hongge Hu, Yanming Huang, Fang Yu, Bin Lou

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引用次数: 0

Abstract

Purpose: In the current work, we investigated the potential protective function of MANF against the degeneration of RP in S334ter-3 rats and the mechanism underlying these effects.

Methods: The left eye of rat was injected with MANF, and the right eye injected PBS as a control. The levels of those ER stress associated proteins were determined by western blot analysis.

Results: First, those ER stress associated proteins were detected in S334ter-3 rats. Among these proteins, a decrease in the expression of PERK was only showed from PD 6 to PD 12. Surprisingly, a dramatic increase of ATF6 was induced by injection MANF. Then, the expression of ATF6 protein declined rapidly to the control eye. Furthermore, a remarkable increase in the expression of CHOP was examined at 6 h, which peaked at 12 h and maintained the level until 48 h. At the same time, CHOP was detected in Müller cells but not in photoreceptor; nevertheless, photoreceptor survival clearly improved. The increasing expressions of CHOP and ATF6 in SD rats were similar to the changes in S33ter-3 rats after injection MANF.

Conclusions: These results indicate that MANF may play an important role in protecting photoreceptor degeneration in RP.

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目的：在本研究中，我们探讨了MANF对S334ter-3大鼠RP变性的潜在保护功能及其作用机制：方法：给大鼠左眼注射 MANF，右眼注射 PBS 作为对照。方法：给大鼠左眼注射 MANF，右眼注射 PBS 作为对照，用 Western 印迹分析法检测ER 应激相关蛋白的水平：结果：首先，在S334ter-3大鼠体内检测到了ER应激相关蛋白。首先，在 S334ter-3 大鼠体内检测到了ER应激相关蛋白。令人惊讶的是，注射 MANF 会诱导 ATF6 的急剧增加。随后，ATF6 蛋白的表达迅速下降至对照眼。与此同时，在Müller细胞中检测到了CHOP，但在感光细胞中却没有检测到；尽管如此，感光细胞的存活率明显提高。SD大鼠CHOP和ATF6表达量的增加与S33ter-3大鼠注射MANF后的变化相似：这些结果表明，MANF可能在保护RP光感受器变性方面发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ophthalmic Genetics 医学-眼科学

CiteScore

2.40

自引率

8.30%

发文量

126

审稿时长

>12 weeks

期刊介绍： Ophthalmic Genetics accepts original papers, review articles and short communications on the clinical and molecular genetic aspects of ocular diseases.