{"title":"Investigating the Pathology-Related Astroglial Plasticity in the Human Cerebral Cortex.","authors":"Swetlana Sirko, Patrizia Della Vecchia","doi":"10.1007/978-1-0716-4366-2_11","DOIUrl":null,"url":null,"abstract":"<p><p>Evaluation of astrocyte proliferation in human brain pathology plays a key role in the diagnostic and prognostic assessment of diseases, especially in cases of benign or malignant brain neoplasms. However, the proliferative potential of nonneoplastic astrocytes within the affected human brain parenchyma has not been well defined. Given the beneficial functions of proliferating reactive astrocytes for brain repair in experimental models of stroke and trauma, investigating the context-specific potential of human astrocytes to proliferate will be a major step forward in exploiting their roles in the genesis, progression, and outcome of neurological diseases in patients. Here, we describe a step-by-step protocol for immunofluorescent staining and neurosphere-forming assay tailored to uncover the astrocyte proliferation and neural stem cell properties in samples of human brain tissue obtained during neurosurgical resections or in a small brain biopsy. This protocol allows for reliable assessment and evaluation of adoptive astrocyte plasticity in the context with various neuropathological conditions in the human brain.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2896 ","pages":"147-164"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-0716-4366-2_11","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Evaluation of astrocyte proliferation in human brain pathology plays a key role in the diagnostic and prognostic assessment of diseases, especially in cases of benign or malignant brain neoplasms. However, the proliferative potential of nonneoplastic astrocytes within the affected human brain parenchyma has not been well defined. Given the beneficial functions of proliferating reactive astrocytes for brain repair in experimental models of stroke and trauma, investigating the context-specific potential of human astrocytes to proliferate will be a major step forward in exploiting their roles in the genesis, progression, and outcome of neurological diseases in patients. Here, we describe a step-by-step protocol for immunofluorescent staining and neurosphere-forming assay tailored to uncover the astrocyte proliferation and neural stem cell properties in samples of human brain tissue obtained during neurosurgical resections or in a small brain biopsy. This protocol allows for reliable assessment and evaluation of adoptive astrocyte plasticity in the context with various neuropathological conditions in the human brain.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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