Dietary Qiwenghuangbo powder–enriched Limosilactobacillus reuteri protects the intestinal epithelium and alleviates inflammation via a strain-specific mechanism

Q1 Health Professions

Animal models and experimental medicine Pub Date : 2025-03-20 DOI:10.1002/ame2.70016

Qian Xu, Lei Xue, Zhenhua Wu, Shuaishuai Kang, Jia Li, Yifan Wu, Yujun Wu, Jinbiao Zhao, Rujuan Wu, Huiyuan Lv, Junjun Wang, Dandan Han

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Abstract

Background

Qiwenghuangbo powder (QP), composed of Astragalus, Phellodendron chinensis, and Radix pulsatilla, is a traditional Chinese herbal formula, but its effects on weaned piglets remained unclear.

Methods

Weaned piglets fed with 0.5 kg/t QP (QP1), 1 kg/t QP (QP2), low-zinc oxide (ZnO; negative control), and high-ZnO (positive control) diets in two phases, respectively, and the growth performance, intestinal morphology, cytokines, and microbial communities were profiled. The mouse models of colitis induced by Citrobacter rodentium and dextran sulfate sodium (DSS) were employed to elucidate the potential role of QP-fed enriched key species.

Results

Dietary 1.0 kg/t QP alleviated diarrhea and inflammation and improved intestinal development and growth performance of weaned piglets. Moreover, this dietary intervention notably altered microbiota composition, characterized by the enrichment of Limosilactobacillus reuteri. Furthermore, out of three isolated L. reuteri, two strains could alleviate pathogen infection and intestinal inflammation, respectively. Specifically, the anti-inflammatory effect of one strain was achieved by promoting the colonization resistance of C. rodentium as significantly reduced pathogen loads. The other strain mitigated DSS-induced colitis by enhancing the goblet cell function and inhibiting the secretion of pro-inflammatory cytokines, particularly interleukin-1β (IL-1ß) and tumor necrosis factor-α (TNF-α).

Conclusions

Dietary QP improved the growth performance and intestinal health of weaned piglets by promoting the colonization of L. reuteri. The isolated commensal L. reuteri control colitis in a strain-specific mechanism, highlighting the potential of QP and L. reuteri in providing evidence for gut health promotion.

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饲粮中添加七温黄波粉可通过菌株特异性机制保护肠上皮并减轻炎症。

背景：芪温黄波散是一种由黄芪、黄柏和白头翁组成的传统中药配方，但其对断奶仔猪的影响尚不清楚。方法：断奶仔猪分别饲喂0.5 kg/t QP （QP1）、1 kg/t QP （QP2）、低氧化锌（ZnO）；分别饲喂阴性对照和高氧化锌（阳性对照）两期饲料，观察生长性能、肠道形态、细胞因子和微生物群落。采用鼠柠檬酸杆菌（Citrobacter rodentium）和葡聚糖硫酸钠（dextran sulfate sodium， DSS）诱导的小鼠结肠炎模型，探讨qp喂养的富集关键菌种的潜在作用。结果：饲粮1.0 kg/t QP可减轻断奶仔猪腹泻和炎症，改善肠道发育和生长性能。此外，这种饮食干预显著改变了微生物群组成，其特征是罗伊氏乳杆菌的富集。此外，在3株分离的罗伊氏乳杆菌中，有2株分别具有减轻病原体感染和肠道炎症的作用。具体来说，一种菌株的抗炎作用是通过显著降低病原体负荷来促进C. rodentium的定植抗性来实现的。另一种菌株通过增强杯状细胞功能和抑制促炎细胞因子的分泌，特别是白细胞介素-1β （IL-1ß）和肿瘤坏死因子-α (TNF-α)，减轻了dss诱导的结肠炎。结论：饲粮中添加QP可促进罗伊氏乳杆菌的定植，从而改善断奶仔猪的生长性能和肠道健康。分离的共生罗伊氏乳杆菌以菌株特异性机制控制结肠炎，突出了QP和罗伊氏乳杆菌在促进肠道健康方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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