{"title":"Spheroid Invasion Assay of Melanoma Cells by Hanging Drop Technique.","authors":"Asiye Busra Boz Er, Ceren Sumer","doi":"10.1007/7651_2025_615","DOIUrl":null,"url":null,"abstract":"<p><p>The development of more physiologically relevant cancer models has led to the increased adoption of three-dimensional (3D) cell culture systems, such as tumor spheroids, which more accurately replicate the complex tumor microenvironment compared to traditional two-dimensional (2D) cultures. This study utilizes the hanging drop technique to generate melanoma spheroids from A375 and SK-MEL28 cell lines, including both parental and drug-resistant variants. These spheroids were embedded in Matrigel and treated with vemurafenib, cilengitide, or their combination to evaluate the effects on invasion. The combination therapy significantly reduced invasion, particularly in resistant SK-MEL28 spheroids. These findings underscore the importance of 3D spheroid models in studying drug efficacy and resistance mechanisms in cancer.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/7651_2025_615","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
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Abstract
The development of more physiologically relevant cancer models has led to the increased adoption of three-dimensional (3D) cell culture systems, such as tumor spheroids, which more accurately replicate the complex tumor microenvironment compared to traditional two-dimensional (2D) cultures. This study utilizes the hanging drop technique to generate melanoma spheroids from A375 and SK-MEL28 cell lines, including both parental and drug-resistant variants. These spheroids were embedded in Matrigel and treated with vemurafenib, cilengitide, or their combination to evaluate the effects on invasion. The combination therapy significantly reduced invasion, particularly in resistant SK-MEL28 spheroids. These findings underscore the importance of 3D spheroid models in studying drug efficacy and resistance mechanisms in cancer.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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