Evolution and amplification of the trehalose-6-phosphate synthase gene family in Theaceae.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2025-03-19 DOI:10.1186/s12864-025-11475-3

Zaibao Zhang, Tao Xiong, Kejia Li, Kexin Huang, Chunxia Liao, Guangqu Liu

{"title":"Evolution and amplification of the trehalose-6-phosphate synthase gene family in Theaceae.","authors":"Zaibao Zhang, Tao Xiong, Kejia Li, Kexin Huang, Chunxia Liao, Guangqu Liu","doi":"10.1186/s12864-025-11475-3","DOIUrl":null,"url":null,"abstract":"Background: Trehalose-6-phosphate synthase (TPS) is an essential enzyme involved in the production of trehalose, and the genes associated with TPS are crucial for various processes such as growth, development, defense mechanisms, and resistance to stress. However, there has been no documentation regarding the evolution and functional roles of the TPS gene family within Theaceae.Results: Here, we uncovered the lineage-specific evolution of TPS genes in Theaceae. A total of 102 TPS genes were discovered across ten Theaceae species with sequenced genomes. Consistent with the previous classification, our phylogenetic analysis indicated that the TPS genes in Theaceae can be categorized into two primary subfamilies and six distinct clades (I, II-1, II-2, II-3, II-4, II-5), with clade I containing a greater number of introns compared to those found in clade II. Segmental duplication served as the main catalyst for the evolution of TPS genes within Theaceae, and numerous TPS genes exhibited inter-species synteny among various Theaceae species. Most of the TPS genes were ubiquitously expressed, and expression divergence of TPS paralogous pairs was observed. The cis-acting elements found in TPS genes indicated their involvement in responses to phytohormones and stress.Conclusion: This research enhanced our understanding of the lineage-specific evolution of the TPS gene family in Theaceae and offered important insights for future functional analyses.","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":"26 1","pages":"273"},"PeriodicalIF":3.5000,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921518/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Genomics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12864-025-11475-3","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Trehalose-6-phosphate synthase (TPS) is an essential enzyme involved in the production of trehalose, and the genes associated with TPS are crucial for various processes such as growth, development, defense mechanisms, and resistance to stress. However, there has been no documentation regarding the evolution and functional roles of the TPS gene family within Theaceae.

Results: Here, we uncovered the lineage-specific evolution of TPS genes in Theaceae. A total of 102 TPS genes were discovered across ten Theaceae species with sequenced genomes. Consistent with the previous classification, our phylogenetic analysis indicated that the TPS genes in Theaceae can be categorized into two primary subfamilies and six distinct clades (I, II-1, II-2, II-3, II-4, II-5), with clade I containing a greater number of introns compared to those found in clade II. Segmental duplication served as the main catalyst for the evolution of TPS genes within Theaceae, and numerous TPS genes exhibited inter-species synteny among various Theaceae species. Most of the TPS genes were ubiquitously expressed, and expression divergence of TPS paralogous pairs was observed. The cis-acting elements found in TPS genes indicated their involvement in responses to phytohormones and stress.

Conclusion: This research enhanced our understanding of the lineage-specific evolution of the TPS gene family in Theaceae and offered important insights for future functional analyses.

查看原文本刊更多论文

山茶科海藻糖-6-磷酸合酶基因家族的进化与扩增。

背景：海藻糖-6-磷酸合成酶（trehalose -6-phosphate synthase， TPS）是海藻糖合成的重要酶，其相关基因在海藻糖的生长发育、防御机制和抗逆性等过程中起着至关重要的作用。然而，目前还没有关于山茶科TPS基因家族的进化和功能作用的文献。结果：本研究揭示了山茶科植物TPS基因的谱系特异性进化。在10种山茶科植物中共发现102个TPS基因。与之前的分类一致，我们的系统发育分析表明，山茶科的TPS基因可分为两个主要亚科和六个不同的分支（I, II-1, II-2, II-3, II-4, II-5），其中分支I含有更多的内含子。部分重复是山茶科TPS基因进化的主要催化剂，许多TPS基因在不同山茶科物种间表现出种间共生性。大部分TPS基因普遍表达，并观察到TPS同源对的表达差异。在TPS基因中发现的顺式作用元件表明它们参与了对植物激素和胁迫的反应。结论：本研究增强了我们对山茶科植物TPS基因家族的谱系特异性进化的认识，并为进一步的功能分析提供了重要的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.