Transcriptional response of salinity stress in red claw crab Uca arcuata

IF 2.8 2区生物学 Q1 MARINE & FRESHWATER BIOLOGY

Frontiers in Marine Science Pub Date : 2025-03-19 DOI:10.3389/fmars.2025.1575833

Bing Wang, Shaolei Sun, Feijun Zhang, Zhiqiang Han

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引用次数: 0

Abstract

This research identified functional genes and regulatory pathways of salinity stress in red claw crab Uca arcuata, which a globally distributed intertidal species inhabiting saline ecosystems, providing data and theoretical basis for the protection and utilization of U. arcuata in global climate change. In this study, we performed RNA sequencing on U. arcuata gill tissues exposed to salinity gradients (15‰, 25‰ control, and 35‰) to profile transcriptional responses. A total of 63.83 GB of high-quality clean reads were generated, yielding 125,462 unigenes with robust assembly metrics (N50 = 969 bp; mean length = 688 bp). The transcriptome analysis predicted 101,280 coding sequences (CDSs) and 52,706 simple sequence repeats (SSRs). Compared with the control group, the high-salinity group obtained 52 differentially expressed genes (DEGs), with 36 upregulated and 16 downregulated genes. The low-salinity group obtained 1,035 DEGs, with 780 upregulated and 255 downregulated genes. GO analysis showed a significant enrichment of DEGs in signal transduction, enzymatic activity, and binding. KEGG analysis showed that most DEGs were associated with signaling pathways and metabolism. APOA1, APOA2, GPX and GST were specific genes related to salinity adaptation. Five DEGs were randomly selected for quantitative RT-PCR validation, and the results demonstrated that the transcriptome data are highly reliable. However, we did not delve into the key functional genes and their regulatory mechanisms. Joint analysis of the genome and transcriptome of U. arcuata should conduct in the future, and comprehensively elucidate its adaptation mechanism to salinity fluctuations.

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来源期刊

Frontiers in Marine Science Agricultural and Biological Sciences-Aquatic Science

CiteScore

5.10

自引率

16.20%

发文量

2443

审稿时长

14 weeks

期刊介绍： Frontiers in Marine Science publishes rigorously peer-reviewed research that advances our understanding of all aspects of the environment, biology, ecosystem functioning and human interactions with the oceans. Field Chief Editor Carlos M. Duarte at King Abdullah University of Science and Technology Thuwal is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, policy makers and the public worldwide. With the human population predicted to reach 9 billion people by 2050, it is clear that traditional land resources will not suffice to meet the demand for food or energy, required to support high-quality livelihoods. As a result, the oceans are emerging as a source of untapped assets, with new innovative industries, such as aquaculture, marine biotechnology, marine energy and deep-sea mining growing rapidly under a new era characterized by rapid growth of a blue, ocean-based economy. The sustainability of the blue economy is closely dependent on our knowledge about how to mitigate the impacts of the multiple pressures on the ocean ecosystem associated with the increased scale and diversification of industry operations in the ocean and global human pressures on the environment. Therefore, Frontiers in Marine Science particularly welcomes the communication of research outcomes addressing ocean-based solutions for the emerging challenges, including improved forecasting and observational capacities, understanding biodiversity and ecosystem problems, locally and globally, effective management strategies to maintain ocean health, and an improved capacity to sustainably derive resources from the oceans.