Kinetics of zoospores approaching a root using a microfluidic device.

IF 2.2 3区 物理与天体物理 Q2 PHYSICS, FLUIDS & PLASMAS
C Cohen, F X Gauci, X Noblin, E Galiana, A Attard, P Thomen
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引用次数: 0

Abstract

Phytophthora species are plant pathogens that cause considerable damage to agrosystems and ecosystems, and have a major impact on the economy. Infection occurs when their biflagellate zoospores move and reach a root on which they aggregate. However, the communication between the plant and the zoospores and how this communication modifies the behavior of the swimming zoospores is not fully understood. Here we use a microfluidic device incorporating a growing Arabidopsis thaliana root to study the real-time kinetics of Phytophthora parasitica zoospores approaching the root and accumulating (or aggregating) around a specific area called the elongation zone. We show that zoospore kinetics are modified only below a distance of a few hundred microns from the aggregation center, with a decrease in velocity coupled to an increase in the number of turns taken. Furthermore, we show that the rate of aggregation is constant throughout a one-hour experiment, and is dependent on zoospore density. This rate is consistent with the fact that zoospores randomly encounter the region close to the elongation zone, a result compatible with an absence of attraction beyond a few hundred microns. Finally, we show that in our configuration, this absence of attraction can be explained by a residual flow responsible for limiting the diffusion of the signal supposedly emitted by the root to a boundary layer of a few hundred microns.

利用微流体装置研究游动孢子接近根的动力学。
疫霉是一种植物病原体,对农业系统和生态系统造成相当大的损害,并对经济产生重大影响。当它们的双鞭毛游动孢子移动并到达它们聚集的根时,感染就发生了。然而,植物和游动孢子之间的交流以及这种交流如何改变游动孢子的行为尚不完全清楚。在这里,我们使用一个含有生长中的拟南芥根的微流体装置来研究疫霉菌游动孢子接近根并在一个称为伸长区的特定区域周围积累(或聚集)的实时动力学。我们表明,游动孢子动力学仅在距离聚集中心几百微米的距离以下被改变,速度的降低与所采取的次数的增加相耦合。此外,我们表明,在一个小时的实验中,聚集率是恒定的,并且取决于游动孢子的密度。这一速率与游动孢子随机遇到靠近伸长区区域的事实是一致的,这一结果与几百微米以外的吸引力缺失相一致。最后,我们表明,在我们的结构中,这种吸引力的缺失可以用残余流来解释,残余流负责限制根发出的信号扩散到几百微米的边界层。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Physical Review E
Physical Review E PHYSICS, FLUIDS & PLASMASPHYSICS, MATHEMAT-PHYSICS, MATHEMATICAL
CiteScore
4.50
自引率
16.70%
发文量
2110
期刊介绍: Physical Review E (PRE), broad and interdisciplinary in scope, focuses on collective phenomena of many-body systems, with statistical physics and nonlinear dynamics as the central themes of the journal. Physical Review E publishes recent developments in biological and soft matter physics including granular materials, colloids, complex fluids, liquid crystals, and polymers. The journal covers fluid dynamics and plasma physics and includes sections on computational and interdisciplinary physics, for example, complex networks.
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