FAM60A promotes proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin signaling pathway.

IF 1.5 4区 医学 Q4 ONCOLOGY
Translational cancer research Pub Date : 2025-02-28 Epub Date: 2025-02-26 DOI:10.21037/tcr-24-1608
Zhikun Dong, Shuwen Jin, Kan Tang, Xiaomei Li, Yonglin Chen
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引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is one of the most detrimental tumors to human health. Although multimodal therapeutic approaches can improve patient survival rates, the prognosis for advanced-stage patients remains poor. It has been reported that family with sequence similarity 60, member A (FAM60A), a component of the SIN3 transcription regulator family member A (SIN3A)/histone deacetylase (HDAC) complex, plays a significant role in tumorigenesis. However, the precise function and mechanisms of action of FAM60A in CRC have not been fully elucidated. In this study, we aim to further delineate the role of FAM60A in CRC by assessing the protein expression levels of FAM60A and β-catenin in CRC tissues and to explore the potential mechanisms by which FAM60A may promote CRC cell proliferation and invasion through a suite of cellular assays.

Methods: Tumor tissues of 195 CRC patients and 65 adjacent non-neoplastic tissues were collected to construct tissue microarrays. The expression levels of FAM60A, c-Myc, cyclin D1, and β-catenin were detected using immunohistochemistry (IHC) staining, and the relationship between the results and the patients' clinicopathological characteristics and prognosis was analyzed. HCT116 and HT-29 cell lines with overexpression/knockdown of FAM60A were constructed. Western blot (WB) was used to detect the protein expression of FAM60A and β-catenin. Cell proliferation, apoptosis rate, cell cycle, and cell migration and invasion abilities were assessed using cell counting kit-8 (CCK-8) assay, flow cytometry, wound healing assay, and transwell assay, respectively.

Results: FAM60A demonstrated elevated expression in CRC tissues and was positively correlated with tumor infiltration depth, Ki67 proliferation index, and poor prognosis in patients. A positive correlation was observed between FAM60A and the expression of β-catenin, c-Myc, and cyclin D1, and patients with co-expression of FAM60A and β-catenin had a significantly higher rate of distant metastasis. The knockdown of FAM60A markedly reduced the proliferation, migration, and invasive capabilities of HCT116 cells, induced cell cycle arrest, and enhanced apoptosis, whereas its overexpression produced the converse effects. In HT-29 cells, FAM60A knockdown also reduced cell proliferation and impaired wound healing, with overexpression showing opposing outcomes. WB analysis revealed that modulation of FAM60A influenced β-catenin protein levels, suggesting a regulatory link between the two proteins.

Conclusions: FAM60A may be a key regulator factor that modulates proliferation and invasion in CRC cells via the Wnt/β-catenin signaling pathway. Elevated FAM60A expression is associated with an adverse prognosis in CRC, underscoring its potential as a prognostic biomarker.

FAM60A通过调节Wnt/β-catenin信号通路促进结直肠癌细胞的增殖和侵袭。
背景:结直肠癌是危害人类健康最严重的肿瘤之一。虽然多模式治疗方法可以提高患者的生存率,但晚期患者的预后仍然很差。据报道,序列相似度为60的家族成员A (FAM60A)是SIN3转录调节因子家族成员A (SIN3A)/组蛋白去乙酰化酶(HDAC)复合物的一个组成部分,在肿瘤发生中起重要作用。然而,FAM60A在CRC中的确切功能和作用机制尚未完全阐明。在本研究中,我们旨在通过评估FAM60A和β-catenin在结直肠癌组织中的蛋白表达水平来进一步描述FAM60A在结直肠癌中的作用,并通过一系列细胞实验探索FAM60A促进结直肠癌细胞增殖和侵袭的潜在机制。方法:收集195例结直肠癌患者的肿瘤组织和65例癌旁非肿瘤组织,构建组织芯片。采用免疫组化(IHC)染色检测FAM60A、c-Myc、cyclin D1、β-catenin的表达水平,并分析其与患者临床病理特征及预后的关系。构建FAM60A过表达/低表达的HCT116和HT-29细胞系。Western blot (WB)检测FAM60A和β-catenin的蛋白表达。分别采用细胞计数试剂盒-8 (CCK-8)法、流式细胞术、伤口愈合法和transwell法评估细胞增殖、凋亡率、细胞周期、细胞迁移和侵袭能力。结果:FAM60A在结直肠癌组织中表达升高,且与肿瘤浸润深度、Ki67增殖指数、患者预后不良呈正相关。FAM60A与β-catenin、c-Myc、cyclin D1表达呈正相关,且FAM60A与β-catenin共表达的患者远端转移率明显较高。FAM60A的敲低显著降低HCT116细胞的增殖、迁移和侵袭能力,诱导细胞周期阻滞,增强细胞凋亡,而其过表达则产生相反的作用。在HT-29细胞中,FAM60A敲低也会减少细胞增殖和损伤伤口愈合,而过表达则显示相反的结果。WB分析显示FAM60A的调节影响了β-catenin蛋白水平,表明这两种蛋白之间存在调节联系。结论:FAM60A可能是通过Wnt/β-catenin信号通路调控结直肠癌细胞增殖和侵袭的关键调控因子。FAM60A表达升高与结直肠癌的不良预后相关,强调了其作为预后生物标志物的潜力。
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来源期刊
CiteScore
2.10
自引率
0.00%
发文量
252
期刊介绍: Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.
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