Oncogenic role and prognostic significance of PIMREG in melanoma.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2025-02-28 Epub Date: 2025-02-26 DOI:10.21037/tcr-24-1861

Xiao Wei, Yujia Jiang, Tianxiang Xia, Jun Du

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引用次数: 0

Abstract

Background: Phosphatidylinositol binding clathrin assembly protein interacting mitotic regulator (PIMREG) plays a significant role in metaphase-to-anaphase transition in cell cycle. Its aberrant expression has been reported to be in correlation with the development of several tumors. However, its role in melanoma remains unknown. This study aimed to investigate the diagnostic and prognostic roles of PIMREG in skin cutaneous melanoma (SKCM).

Methods: The expression levels of PIMREG were analyzed in SKCM using datasets downloaded from The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Gene Expression Omnibus (GEO). The diagnostic accuracy was assessed using the receiver operating characteristic (ROC) curve. PIMREG was correlated to the functional states of SKCM cells using CancerSEA. Additionally, a protein-protein interaction network was constructed using STRING (https://cn.string-db.org), and hub genes were identified using Cytoscape. Enrichment analysis through Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) was utilized to explore the potential functions of PIMREG. The single-sample GSEA (ssGSEA) method was employed to investigate the correlation between PIMREG expression and the level of immune infiltration in SKCM. Drug sensitivity and resistance were analyzed using GSCALite and Cellminer.

Results: The expression of PIMREG was significantly higher in SKCM tissues. Its overexpression correlated with poor survival outcome in melanoma patients. ROC analysis also revealed that PIMREG had high diagnostic potential, with area under the ROC curve (AUC) value of 0.874. Multivariate regression also identified PIMREG could serve as an independent diagnostic indicator for SKCM. Using the web tool of CancerSEA, we demonstrated that PIMREG is involved in cell cycle, DNA repair, DNA damage, epithelial-mesenchymal transition (EMT), invasion, and proliferation. Functional enrichment analysis revealed that PIMREG might be correlated with some biological processes (BPs) and important pathways related to cancer, including Wnt signaling and epidermis development.

Conclusions: PIMREG is a promising diagnostic and prognostic biomarker and may be regarded as a possible therapeutic target for SKCM.

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PIMREG在黑色素瘤中的致瘤作用及预后意义。

背景：磷脂酰肌醇结合网格蛋白组装蛋白相互作用有丝分裂调节因子（PIMREG）在细胞周期中期向后期转变中起着重要作用。据报道，它的异常表达与几种肿瘤的发展有关。然而，它在黑色素瘤中的作用尚不清楚。本研究旨在探讨PIMREG在皮肤黑色素瘤（SKCM）中的诊断和预后作用。方法：利用从The Cancer Genome Atlas （TCGA）、Gene - types - tissue expression （GTEx）和Gene expression Omnibus （GEO）下载的数据集，分析PIMREG在SKCM中的表达水平。采用受试者工作特征（ROC）曲线评估诊断准确性。使用CancerSEA检测PIMREG与SKCM细胞功能状态的相关性。此外，利用STRING （https://cn.string-db.org）构建了蛋白相互作用网络，并利用Cytoscape鉴定了枢纽基因。通过基因本体（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）进行富集分析，探索PIMREG的潜在功能。采用单样本GSEA （ssGSEA）法研究PIMREG表达与SKCM免疫浸润水平的相关性。采用GSCALite和Cellminer进行药敏和耐药分析。结果：PIMREG在SKCM组织中的表达明显增高。它的过表达与黑色素瘤患者的生存预后差相关。ROC分析也显示PIMREG具有较高的诊断潜力，其ROC曲线下面积（AUC）值为0.874。多因素回归分析表明，PIMREG可作为SKCM的独立诊断指标。利用CancerSEA网络工具，我们证实了PIMREG参与细胞周期、DNA修复、DNA损伤、上皮-间质转化（epithelial-mesenchymal transition， EMT）、侵袭和增殖。功能富集分析显示，PIMREG可能与Wnt信号和表皮发育等肿瘤相关的一些生物学过程和重要通路相关。结论：PIMREG是一种有前景的诊断和预后生物标志物，可能被视为SKCM可能的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.