Jia-Yu Tao, Jun Zhu, Yu-Qiong Gao, Min Jiang, Hong Yin
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引用次数: 0
Abstract
Background and objective: The conventional in vitro research on tumor mechanisms is typically based on two-dimensional (2D) culture of tumor cells, which has many limitations in replicating in vivo tumorigenesis processes. In contrast, the three-dimensional (3D) bioprinting has paved the way for the construction of more biomimetic in vitro tumor models. This article comprehensively elucidates the features of 3D bioprinting and meticulously summarizes its applications in several selected tumors, aiming to offer valuable insights for future relevant studies.
Methods: A literature search was conducted in the databases of PubMed and Web of Science for articles on 3D bioprinting for in vitro tumor model construction.
Key content and findings: This article introduces various 3D bioprinting technologies for in vitro tumor model construction, focusing on their pros and cons, principles, and protocols. Several in vitro tumor models are presented, detailing their utility in tumorigenesis research and their constraints. To date, 3D bioprinting has been widely applied in oncology, addressing the limitation of traditional 2D tumor cell culture in replicating tumor microenvironment (TME).
Conclusions: Advanced 3D bioprinting technology accurately replicates the complex TME and the heterogeneity of intratumor structures, enabling further in vitro tumor studies. It significantly fuels our understanding of tumor pathophysiology and offers new hope for cancer patients.
背景与目的:传统的肿瘤机制体外研究通常是基于肿瘤细胞的二维培养,这在复制体内肿瘤发生过程中存在许多局限性。相比之下,三维(3D)生物打印为构建更多的仿生体外肿瘤模型铺平了道路。本文全面阐述了3D生物打印的特点,并对其在几种肿瘤中的应用进行了精心总结,旨在为今后的相关研究提供有价值的见解。方法:在PubMed和Web of Science数据库中检索3D生物打印用于体外肿瘤模型构建的相关文章。主要内容和发现:本文介绍了用于体外肿瘤模型构建的各种生物3D打印技术,重点介绍了它们的优缺点、原理和方案。介绍了几种体外肿瘤模型,详细介绍了它们在肿瘤发生研究中的应用及其局限性。目前,3D生物打印技术已广泛应用于肿瘤领域,解决了传统2D肿瘤细胞培养在复制肿瘤微环境(TME)方面的局限性。结论:先进的生物3D打印技术准确地复制了复杂的TME和肿瘤内结构的异质性,为进一步的体外肿瘤研究提供了可能。它极大地推动了我们对肿瘤病理生理学的理解,并为癌症患者带来了新的希望。
期刊介绍:
Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.