A novel necroptosis-related miRNA signature for predicting the prognosis of esophageal cancer and immune infiltration analysis.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2025-02-28 Epub Date: 2025-02-26 DOI:10.21037/tcr-24-1532

Miao Zhang, Shaoran Song, Bo Wang, Yangyang Shang, Peijun Liu, Juan Li

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引用次数: 0

Abstract

Background: The prognostic value of necroptosis-related microRNAs (miRNAs), which are important in tumorigenesis and development, remains unclear. Therefore, we aimed to screen prognostic necroptosis-related miRNAs in esophageal cancer (EC).

Methods: Nine necroptosis-related miRNA expression profiles and associated clinical data of EC patients were obtained from The Cancer Genome Atlas (TCGA) database. The relationships between necroptosis-related miRNAs and overall survival (OS) were determined via Cox regression model analysis. Target genes of the miRNAs were investigated in TargetScan, miRDB, and miRTarBase. The biological functions of these genes were evaluated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. For the most significant correlation between miR-425-5p expression and the survival of EC patients, the effect of miR-425-5p on necroptosis was explored in EC cells. The relationship between targeted gene expression and immune infiltration was also analyzed and validated.

Results: Hsa-miR-425-5p, hsa-miR-500a-3p, hsa-miR-7-5p and hsa-miR-200a-5p were selected for the construction of a prognostic signature based on their correlation with the survival of EC patients. EC patients were divided into high- and low-risk groups according to the median value of the risk score. Patients in the high-risk group tended to have higher death rates than those in the low-risk group (P<0.05). The risk score was an independent prognostic indicator for the OS of EC patients [hazard ratio (HR) >1, P<0.05]. The prognostic model had good predictive efficiency. The genes targeted by necroptosis-related miRNAs were significantly enriched in apoptosis etc. The inhibition of miR-425-5p promoted necroptosis in EC cells by targeting branched chain amino acid transaminase 1 (BCAT1). The expression level of BCAT1 was significantly correlated with immune infiltration.

Conclusions: A necroptosis-related four-miRNA model was constructed successfully to predict the potential value of the four miRNAs in the prognosis of EC, which can be conducive to promoting the therapeutic effect on EC.

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一种预测食管癌预后和免疫浸润分析的新型坏死相关miRNA特征。

背景：坏死相关的microRNAs （miRNAs）在肿瘤发生和发展中起重要作用，其预后价值尚不清楚。因此，我们旨在筛选食管癌（EC）预后坏死性坏死相关的mirna。方法：从癌症基因组图谱（TCGA）数据库中获取EC患者9例坏死相关miRNA表达谱及相关临床资料。通过Cox回归模型分析确定坏死相关mirna与总生存期（OS）的关系。在TargetScan、miRDB和miRTarBase中研究mirna的靶基因。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）分析评估这些基因的生物学功能。对于miR-425-5p表达与EC患者生存之间最显著的相关性，我们探索了miR-425-5p对EC细胞坏死性坏死的影响。分析并验证了靶基因表达与免疫浸润的关系。结果：根据Hsa-miR-425-5p、hsa-miR-500a-3p、hsa-miR-7-5p和hsa-miR-200a-5p与EC患者生存的相关性，选择Hsa-miR-425-5p构建预后特征。根据风险评分中位数将EC患者分为高危组和低危组。高危组患者的死亡率往往高于低危组（P1, PBCAT1）。BCAT1表达水平与免疫浸润显著相关。结论：成功构建了坏死相关的4 - mirna模型，预测了4种mirna在EC预后中的潜在价值，有助于提高EC的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.