Hsa_circ_0002346 inhibits proliferation, invasion, and migration of breast cancer cells and promotes apoptosis: A novel potential biomarker for breast cancer.

IF 2.2 4区医学 Q3 ONCOLOGY

Cancer Biomarkers Pub Date : 2024-12-01 Epub Date: 2025-03-17 DOI:10.1177/18758592241297847

Yixin Zhou, Linyi Wang, Wangjin Xu, Jiaxiang An, Guoming Hu, Feilin Cao, Liming Huang

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引用次数: 0

Abstract

BackgroundCircular RNA hsa_circ_0002346 has been implicated in the progression of various tumors, yet the functional role in breast cancer remains poorly understood. This study aimed to investigate the significance of hsa_circ_0002346 in breast cancer (BC).MethodsQuantitative reverse transcriptase polymerase chain reaction assays were performed to detect hsa_circ_0002346 expression in BC cell lins and 27 patients with BC. Then, siRNAs were used to knock down hsa_circ_0002346. And detecting function of downregulated hsa_circ_0002346 by proliferation colony formation, apoptosis assays, wound-healing assays and Transwell assays. Finally, we assess the levels of the EMT-associated proteins.ResultsOur findings suggest that hsa_circ_0002346 levels were significantly downregulated in breast cancer and may play a crucial role in regulating key cellular processes associated with cancer progression. The expression level of hsa_circ_0002346 was correlated with lymph node metastasis. The knockdown of hsa_circ_0002346 resulted in increased tumor cell proliferation, invasion, migration and decreased apoptosis. Additionally, alterations in the expression of EMT-associated proteins further support the hypothesis that hsa_circ_0002346 is implicated in the metastatic processes of breast cancer.Conclusionhsa_circ_0002346 emerges as a promising biomarker for breast cancer and a potential therapeutic target for future treatment strategies.

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Hsa_circ_0002346抑制乳腺癌细胞增殖、侵袭和迁移，促进细胞凋亡：一种新的潜在乳腺癌生物标志物

环状RNA hsa_circ_0002346与多种肿瘤的进展有关，但其在乳腺癌中的功能作用仍知之甚少。本研究旨在探讨hsa_circ_0002346在乳腺癌（BC）中的意义。方法采用逆转录酶聚合酶链反应定量检测hsa_circ_0002346在BC细胞株和27例BC患者中的表达。然后，sirna被用来敲除hsa_circ_0002346。并通过增殖集落形成、细胞凋亡、创面愈合和Transwell实验检测下调hsa_circ_0002346的功能。最后，我们评估emt相关蛋白的水平。结果hsa_circ_0002346水平在乳腺癌中显著下调，可能在调节与癌症进展相关的关键细胞过程中发挥关键作用。hsa_circ_0002346表达水平与淋巴结转移相关。敲低hsa_circ_0002346导致肿瘤细胞增殖、侵袭、迁移增加，凋亡减少。此外，emt相关蛋白表达的改变进一步支持了hsa_circ_0002346参与乳腺癌转移过程的假设。结论hsa_circ_0002346是一种有前景的乳腺癌生物标志物，是未来治疗策略的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Biomarkers ONCOLOGY-

CiteScore

5.20

自引率

3.20%

发文量

195

审稿时长

3 months

期刊介绍： Concentrating on molecular biomarkers in cancer research, Cancer Biomarkers publishes original research findings (and reviews solicited by the editor) on the subject of the identification of markers associated with the disease processes whether or not they are an integral part of the pathological lesion. The disease markers may include, but are not limited to, genomic, epigenomic, proteomics, cellular and morphologic, and genetic factors predisposing to the disease or indicating the occurrence of the disease. Manuscripts on these factors or biomarkers, either in altered forms, abnormal concentrations or with abnormal tissue distribution leading to disease causation will be accepted.