Hsa_circ_0002346 inhibits proliferation, invasion, and migration of breast cancer cells and promotes apoptosis: A novel potential biomarker for breast cancer.

IF 2.2 4区医学 Q3 ONCOLOGY

Cancer Biomarkers Pub Date : 2024-12-01 Epub Date: 2025-03-17 DOI:10.1177/18758592241297847

Yixin Zhou, Linyi Wang, Wangjin Xu, Jiaxiang An, Guoming Hu, Feilin Cao, Liming Huang

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引用次数: 0

Abstract

BackgroundCircular RNA hsa_circ_0002346 has been implicated in the progression of various tumors, yet the functional role in breast cancer remains poorly understood. This study aimed to investigate the significance of hsa_circ_0002346 in breast cancer (BC).MethodsQuantitative reverse transcriptase polymerase chain reaction assays were performed to detect hsa_circ_0002346 expression in BC cell lins and 27 patients with BC. Then, siRNAs were used to knock down hsa_circ_0002346. And detecting function of downregulated hsa_circ_0002346 by proliferation colony formation, apoptosis assays, wound-healing assays and Transwell assays. Finally, we assess the levels of the EMT-associated proteins.ResultsOur findings suggest that hsa_circ_0002346 levels were significantly downregulated in breast cancer and may play a crucial role in regulating key cellular processes associated with cancer progression. The expression level of hsa_circ_0002346 was correlated with lymph node metastasis. The knockdown of hsa_circ_0002346 resulted in increased tumor cell proliferation, invasion, migration and decreased apoptosis. Additionally, alterations in the expression of EMT-associated proteins further support the hypothesis that hsa_circ_0002346 is implicated in the metastatic processes of breast cancer.Conclusionhsa_circ_0002346 emerges as a promising biomarker for breast cancer and a potential therapeutic target for future treatment strategies.

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来源期刊

Cancer Biomarkers ONCOLOGY-

CiteScore

5.20

自引率

3.20%

发文量

195

审稿时长

3 months

期刊介绍： Concentrating on molecular biomarkers in cancer research, Cancer Biomarkers publishes original research findings (and reviews solicited by the editor) on the subject of the identification of markers associated with the disease processes whether or not they are an integral part of the pathological lesion. The disease markers may include, but are not limited to, genomic, epigenomic, proteomics, cellular and morphologic, and genetic factors predisposing to the disease or indicating the occurrence of the disease. Manuscripts on these factors or biomarkers, either in altered forms, abnormal concentrations or with abnormal tissue distribution leading to disease causation will be accepted.