EIF4A3 Promotes Cell Proliferation via CDC5L Upregulation in Human Breast Cancer Cells.

Abstract

Breast cancer is one of the most common diseases affecting women's health. While research on breast cancer has made progress in recent years, it remains a major health concern. Studies have shown that the translation initiation factor EIF4A3 is closely related to the occurrence and development of tumors, but the specific mechanism is still unclear. In this study, we aimed to explore the specific molecular mechanism of EIF4A3 in promoting the malignant process of breast cancer in vivo and in vitro. Our results showed that the expression of EIF4A3 was significantly upregulated in breast cancer, and overexpression of EIF4A3 could accelerate the growth of breast cancer cells. RIP-seq and RIP-RT-qPCR analyses indicated that EIF4A3 can bind to the mRNA of CDC5L and influence its expression. From the catRAPID we predicted that EIF4A3-protein could bind to CDC5L by the 5705-5954 region of CDC5L-mRNA. CDC5L was the downstream effector of EIF4A3. These results suggested that the EIF4A3-CDC5L axis promotes the proliferation of breast cancer cells. This study provides a theoretical basis for understanding the role of EIF4A3 in the malignant process of breast cancer.