CCL4 and MIF: Prognostic Biomarkers for Evaluating the Chemoradiotherapy Response and Prognosis in Patients with ESCC.

IF 3.3 3区 医学 Q2 ONCOLOGY
Journal of Cancer Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI:10.7150/jca.104088
Yuwen Wang, Chunxue Ding, Xiaoying Wei, Yuting Li, Xuyao Yu, Xi Chen, Jifeng Sun, Junhong Zhuang, Shuai Cao, Peng Zhen, Fang Fang, Jiarui Zhang, Jun Wang, Dong Qian, Qingsong Pang
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引用次数: 0

Abstract

Background: Radiotherapy plays a central role in therapeutic strategy of local-advanced oesophageal squamous cell carcinoma. The aim of this study was to investigate cytokine profiles in serum of patients with ESCC and evaluate the potential utility of cytokine markers in predicting CRT response and prognostic prediction. Methods: CCL4, MIF and CXCL8 in the serum samples who were participating in a phase II clinical trial (NCT02959385) were determined. The association between these cytokines and CRT response as well as prognostic prediction were subsequently assessed in ESCC. Subsequently, the results were verified in ESCC tissue and in the Cancer Genome Atals (TCGA) database. Results: The expression of 120 cytokines were evaluated in serum of 4 ESCC patients with excellent CRT-response and 4 patients with CRT-resistance by cytokine microarrays. CCL3, CCL4, MIF, PLAUR and CXCL8 were screened. CCL4, MIF and CXCL8 were further detected by ELISA in other 60 patients enrolled in this study. Upregulation of CCL4, CXCL8 and MIF were observed in patients with excellent CRT-response. Elevated expression of CCL4 and MIF were closely associated with improved PFS and OS outcomes. Similar results were obtained in other 46 ESCC tumor tissues. 82 ESCC patients in TCGA database with increased CCL4 and MIF expression exhibited the favorable immunocyte infiltration and enriched immune response-related pathways, which indicates the preferable tumor immunogenicity. Conclusions: CCL4 and MIF are identified as dependable and prognostic biomarkers for evaluating the response to CRT and prognosis in patients with ESCC.

CCL4和MIF:评估ESCC患者放化疗反应和预后的预后生物标志物
背景:放射治疗在局部晚期食管鳞状细胞癌的治疗策略中起着核心作用。本研究的目的是调查ESCC患者血清中的细胞因子特征,并评估细胞因子标志物在预测CRT反应和预后预测中的潜在效用。方法:检测参加II期临床试验(NCT02959385)的血清样本中的CCL4、MIF和CXCL8。随后在ESCC中评估了这些细胞因子与CRT反应以及预后预测之间的关系。随后,结果在ESCC组织和癌症基因组数据库(TCGA)中得到验证。结果:采用细胞因子芯片技术检测了4例crt反应优异者和4例crt耐药者血清中120种细胞因子的表达。筛选CCL3、CCL4、MIF、PLAUR和CXCL8。另外60例患者的CCL4、MIF和CXCL8也通过ELISA检测。CCL4、CXCL8和MIF在crt应答良好的患者中上调。CCL4和MIF的表达升高与PFS和OS的改善密切相关。在其他46例ESCC肿瘤组织中也获得了类似的结果。TCGA数据库中CCL4和MIF表达升高的82例ESCC患者表现出良好的免疫细胞浸润和丰富的免疫应答相关通路,表明具有较好的肿瘤免疫原性。结论:CCL4和MIF被认为是评估ESCC患者对CRT反应和预后的可靠和预后的生物标志物。
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来源期刊
Journal of Cancer
Journal of Cancer ONCOLOGY-
CiteScore
8.10
自引率
2.60%
发文量
333
审稿时长
12 weeks
期刊介绍: Journal of Cancer is an open access, peer-reviewed journal with broad scope covering all areas of cancer research, especially novel concepts, new methods, new regimens, new therapeutic agents, and alternative approaches for early detection and intervention of cancer. The Journal is supported by an international editorial board consisting of a distinguished team of cancer researchers. Journal of Cancer aims at rapid publication of high quality results in cancer research while maintaining rigorous peer-review process.
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