Role of EhLINE1_ORF1p outside retrotransposition: Downregulation causes growth defects in Entamoeba histolytica.

IF 2.1 4区 生物学 Q2 BIOLOGY
Journal of Biosciences Pub Date : 2025-01-01
Devinder Kaur, Shashi Shekhar Singh, Shraddha Sharma, Sudha Bhattacharya
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引用次数: 0

Abstract

The constitutive expression of EhLINE1_ORF1p in Entamoeba histolytica cells in the absence of retrotransposition suggests that this protein may serve roles beyond retrotransposition. To delve into this possibility, we generated and analyzed transcriptomic data of EhLINE1_ORF1p partial-knockdown cell lines. This analysis unveiled the significance of EhLINE1_ORF1p in the growth of E. histolytica cells. Our investigation of the consequences of EhLINE1_ORF1p partial-knockdown highlighted its impact on the genes involved in ribosome biogenesis, pre-rRNA processing, RNA helicase, and oxidoreductase activity, particularly those associated with amoebiasis. These affected genes are categorized as having high expression and are essential for optimal cell growth. Validation through western blotting confirmed a substantial and pronounced decline in EhLINE1_ORF1p level, and growth kinetics confirmed decline in cell growth. These findings provide compelling evidence supporting the plausible role of EhLINE1_ORF1p in regulating the growth of E. histolytica.

在没有逆转录作用的情况下,EhLINE1_ORF1p在组织溶线虫细胞中的组成型表达表明,该蛋白的作用可能超出了逆转录作用。为了深入研究这种可能性,我们生成并分析了EhLINE1_ORF1p部分敲除细胞系的转录组数据。这项分析揭示了 EhLINE1_ORF1p 在组织溶解虫细胞生长过程中的重要作用。我们对 EhLINE1_ORF1p 部分敲除的后果进行了调查,结果表明它对参与核糖体生物发生、前 RNA 处理、RNA 螺旋酶和氧化还原酶活性的基因产生了影响,尤其是那些与阿米巴病相关的基因。这些受影响的基因被归类为高表达基因,对于细胞的最佳生长至关重要。通过 Western 印迹验证证实了 EhLINE1_ORF1p 水平的显著下降,生长动力学也证实了细胞生长的下降。这些发现提供了令人信服的证据,支持 EhLINE1_ORF1p 在调节溶血性大肠杆菌生长过程中的合理作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Biosciences
Journal of Biosciences 生物-生物学
CiteScore
5.80
自引率
0.00%
发文量
83
审稿时长
3 months
期刊介绍: The Journal of Biosciences is a quarterly journal published by the Indian Academy of Sciences, Bangalore. It covers all areas of Biology and is the premier journal in the country within its scope. It is indexed in Current Contents and other standard Biological and Medical databases. The Journal of Biosciences began in 1934 as the Proceedings of the Indian Academy of Sciences (Section B). This continued until 1978 when it was split into three parts : Proceedings-Animal Sciences, Proceedings-Plant Sciences and Proceedings-Experimental Biology. Proceedings-Experimental Biology was renamed Journal of Biosciences in 1979; and in 1991, Proceedings-Animal Sciences and Proceedings-Plant Sciences merged with it.
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