Genome-wide association study of neuropathological features in Lewy body disease

Abstract

Studies assessing genetic associations with neuropathological features in Lewy body disease (LBD) have been limited to candidate gene investigations, and therefore information is lacking regarding the genetic architecture of the neuropathology of LBD. In the current study, we examined a large series of neuropathologically-confirmed LBD cases (N=980 in the discovery series, N=503 in the replication series) and performed genome-wide association studies (GWAS’s) of 11 different neuropathological outcome measures. The 11 neuropathological outcomes included Braak neurofibrillary tangle (NFT) stage, Thal amyloid phase, LBD subtype, Lewy body (LB) counts in five different brain regions, dorsolateral and ventromedial putaminal tyrosine hydroxylase immunoreactivity (TH-ir), and neuronal loss in the ventrolateral part of the substantia nigra (SN). Associations between variants and outcomes were assessed using regression models appropriate for the nature of the given neuropathological outcome and that were adjusted for age at death, sex, and top principal components of genetic data. In the discovery series, APOE rs429358 (i.e., APOE ε4) was associated with a greater severity of each of Braak NFT stage (OR=3.07, P=2.34 x 10-32), Thal amyloid phase (OR=3.57, P=3.28 x 10-29), and LBD subtype (OR=1.78, P=9.85 x 10-9), with similar findings observed in the independent replication series (Braak NFT stage, OR=2.30, P=2.70 x 10-11; Thal amyloid phase, OR=3.17, P=6.39 x 10-18; LBD subtype, OR=2.68, P=3.85 x 10-10). In the subgroup of cases with lower levels of AD pathology (Braak NFT stage ≤III and Thal amyloid phase ≤2), there was a strong association between APOE rs429358 and LBD subtype in even when adjusting for Braak stage and Thal phase in the discovery series (N=218, OR=2.47, P=0.007) and the replication series (N=141, OR=3.60, P=0.006). Although additional genome-wide significant associations were identified in the discovery series between LINC01581/MCTP2 rs547411734 and lower middle frontal LB counts, between TLE3 rs3743309 and lower cingulate LB counts, and between GRIN2A/ATF7IP2 rs1097915 and lower parahippocampal LB counts, these findings were not observed in the replication series. Our results indicate that the APOE ε4 allele is the most prominent genetic determinant of severity of neuropathology in LBD. These findings represent a key step forward in our understanding of genetic drivers of neuropathological features in LBD. Future studies utilizing meta-analytical approaches will be important to more precisely assess other associations that were not quite genome-wide significant in the discovery series.