Fabrication of porous collagen-stem cells-dexamethasone scaffold as a novel approach for regeneration of mandibular bone defect.

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Oral and Maxillofacial Surgery-Heidelberg Pub Date : 2025-03-12 DOI:10.1007/s10006-025-01353-3

Nader Tanideh, Mobina Sarikhani, Mina Emami, Masih Alipanah, Yasaman Mohammadi, Mohammad Mokhtarzadegan, Akram Jamshidzadeh, Shahrokh Zare, Sajad Daneshi, Ali Feiz, Cambyz Irajie, Aida Iraji

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引用次数: 0

Abstract

Background: Bone defects, particularly in the mandible, pose significant clinical challenges due to the limited regenerative capacity. Effective bone tissue engineering requires biomaterials that promote both osteogenesis and angiogenesis. This study developed an optimized collagen-nano hydroxyapatite scaffold loaded with dexamethasone and stem cells to enhance bone regeneration.

Methods: The scaffold was fabricated using the freeze-dryer method. Characterization was performed using Fourier Transform Infrared Spectroscopy (FTIR), energy-dispersive X-ray (EDX) analysis, and scanning electron microscopy (SEM). Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) were incorporated into the scaffold, and in vitro and in vivo assessments were conducted.

Results: FTIR and EDX analyses confirmed the successful incorporation of nano-hydroxyapatite and dexamethasone. SEM revealed an interconnected porous structure with an average pore size of 28.55 µm. The scaffold loaded with WJ-MSCs significantly enhanced osteocyte and osteoblast populations, leading to improved mandibular bone formation. Histopathological evaluations demonstrated superior osteogenesis and angiogenesis.

Conclusion: The developed porous nanohybrid scaffold shows potential as a promising biomaterial for bone tissue engineering applications.

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胶原-干细胞-地塞米松多孔支架的制备及其在下颌骨缺损修复中的应用。

背景：骨缺损，尤其是下颌骨的骨缺损，由于其再生能力有限，给临床治疗带来了重大挑战。有效的骨组织工程需要既促进成骨又促进血管生成的生物材料。本研究开发了一种优化的胶原-纳米羟基磷灰石支架，负载地塞米松和干细胞，以促进骨再生。方法：采用冷冻干燥法制备支架。采用傅里叶变换红外光谱（FTIR）、能量色散x射线（EDX）分析和扫描电子显微镜（SEM）进行表征。将Wharton’s jelly-derived mesenchymal stem cells （WJ-MSCs）加入到支架中，并进行体外和体内评估。结果：FTIR和EDX分析证实纳米羟基磷灰石与地塞米松的成功结合。扫描电镜显示，其孔隙结构相互连接，平均孔径为28.55µm。负载WJ-MSCs的支架可显著增加成骨细胞和成骨细胞的数量，从而改善下颌骨的形成。组织病理学评估显示其具有良好的成骨和血管生成能力。结论：所制备的多孔纳米复合支架具有良好的骨组织工程应用前景。

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来源期刊

Oral and Maxillofacial Surgery-Heidelberg DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.20

自引率

5.60%

发文量

118

期刊介绍： Oral & Maxillofacial Surgery founded as Mund-, Kiefer- und Gesichtschirurgie is a peer-reviewed online journal. It is designed for clinicians as well as researchers.The quarterly journal offers comprehensive coverage of new techniques, important developments and innovative ideas in oral and maxillofacial surgery and interdisciplinary aspects of cranial, facial and oral diseases and their management. The journal publishes papers of the highest scientific merit and widest possible scope on work in oral and maxillofacial surgery as well as supporting specialties. Practice-oriented articles help improve the methods used in oral and maxillofacial surgery.Every aspect of oral and maxillofacial surgery is fully covered through a range of invited review articles, clinical and research articles, technical notes, abstracts, and case reports. Specific topics are: aesthetic facial surgery, clinical pathology, computer-assisted surgery, congenital and craniofacial deformities, dentoalveolar surgery, head and neck oncology, implant dentistry, oral medicine, orthognathic surgery, reconstructive surgery, skull base surgery, TMJ and trauma.Time-limited reviewing and electronic processing allow to publish articles as fast as possible. Accepted articles are rapidly accessible online.Clinical studies submitted for publication have to include a declaration that they have been approved by an ethical committee according to the World Medical Association Declaration of Helsinki 1964 (last amendment during the 52nd World Medical Association General Assembly, Edinburgh, Scotland, October 2000). Experimental animal studies have to be carried out according to the principles of laboratory animal care (NIH publication No 86-23, revised 1985).