Correlation of thermochemical and spectral characteristics as a method for determining the type of binding of porphyrins to nucleic acids

IF 5.3 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Liquids Pub Date : 2025-03-14 DOI:10.1016/j.molliq.2025.127387

Sabir S. Guseinov, Elena S. Yurina, Natalya Sh. Lebedeva

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引用次数: 0

Abstract

The work is devoted to the analysis and generalization of the thermochemical and spectral characteristics of complexes of porphyrins with synthetic oligonucleotides poly[d(AT)₂], poly[d(GC)₂] and calf thymus DNA of solutions, studied in our laboratory. The interactions of nucleic acids (NA) with cationic porphyrins containing indole (PorIn), caffeine (PorCofe), adenine (PorAd) and guanine (PorGu) residues as substituents were studied by spectrophotometric titration. The calorimetric method was used for measuring the temperature dependences of the heat capacity of solutions of cationic porphyrins, NA, as well as complexes. A correlation relationship was obtained for the temperature dependence of the specific heat capacity of NA-porphyrin-buffer. It was found that there is a correlation between the heat capacity of solutions of the complexes at 25 °C, the ratio of hypochromic effect to the shift of the maximum of absorption of the band Sore and the way of binding of NA to cationic porphyrins. Spectral methods for recording changes in the photophysical properties of chromophore ligands made it possible in most cases to reveal the mechanism of binding of porphyrins to NA. Thermochemical studies provided the evaluation of the structural consequences of the complexation process and clarification of the method of porphyrins binding to NA. The perspectives of joint application of the results of thermochemical and spectral studies to elucidate the structural aspects of the formation of complexes NA-porphyrin are discussed in this paper.

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来源期刊

Journal of Molecular Liquids 化学-物理：原子、分子和化学物理

CiteScore

10.30

自引率

16.70%

发文量

2597

审稿时长

78 days

期刊介绍： The journal includes papers in the following areas: – Simple organic liquids and mixtures – Ionic liquids – Surfactant solutions (including micelles and vesicles) and liquid interfaces – Colloidal solutions and nanoparticles – Thermotropic and lyotropic liquid crystals – Ferrofluids – Water, aqueous solutions and other hydrogen-bonded liquids – Lubricants, polymer solutions and melts – Molten metals and salts – Phase transitions and critical phenomena in liquids and confined fluids – Self assembly in complex liquids.– Biomolecules in solution The emphasis is on the molecular (or microscopic) understanding of particular liquids or liquid systems, especially concerning structure, dynamics and intermolecular forces. The experimental techniques used may include: – Conventional spectroscopy (mid-IR and far-IR, Raman, NMR, etc.) – Non-linear optics and time resolved spectroscopy (psec, fsec, asec, ISRS, etc.) – Light scattering (Rayleigh, Brillouin, PCS, etc.) – Dielectric relaxation – X-ray and neutron scattering and diffraction. Experimental studies, computer simulations (MD or MC) and analytical theory will be considered for publication; papers just reporting experimental results that do not contribute to the understanding of the fundamentals of molecular and ionic liquids will not be accepted. Only papers of a non-routine nature and advancing the field will be considered for publication.