Akkermansia muciniphila and Its Extracellular Vesicles Affect Endocannabinoid System in in vitro Model.

IF 3.6 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Digestion Pub Date : 2025-01-01 Epub Date: 2025-03-13 DOI:10.1159/000543446
Pegah Noori, Fattah Sotoodehnejadnematalahi, Pooneh Rahimi, Seyed Davar Siadat
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引用次数: 0

Abstract

Introduction: Recent studies indicate that the gut microbiota controls the host's immune system. Probiotics use different signaling pathways to regulate intestinal permeability, barrier integrity, and energy balance.

Methods: This research examined how Akkermansia muciniphila and its extracellular vesicles (EVs) impact inflammation and genes related to the endocannabinoid system in the STC-1 cell line through RT-PCR and ELISA assays.

Results: The study's results indicated that EVs had a significant impact on GLP-1 expression compared to the multiplicity of infections (MOI) ratio. Notably, there was a substantial increase in the expression of PYY and GLP-1 genes across all treatments (p < 0.05). Conversely, the expression of CB-1, CB-2, and FAAH genes notably decreased in the STC-1 cell line when treated with MOI 50 of A. muciniphila and an EV concentration of 100 μg/mL (p < 0.05). Both MOI 50 of A. muciniphila and an EV concentration of 100 μg/mL significantly enhanced the expression of the TLR-2 gene. In contrast, EVs at a concentration of 100 μg/mL substantially reduced TLR-4 gene expression. A. muciniphila-derived EVs notably decreased the levels of inflammatory cytokines (TNF-α and IL-6), while increasing IL-10 expression at MOI 100 and an EV concentration of 100 μg/mL. These findings suggest that A. muciniphila and its EVs could regulate the expression of specific genes, serving as targets for maintaining host energy balance.

Conclusions: In summary, this study illustrates that A. muciniphila-derived EVs exhibit anti-inflammatory properties and have the potential to modulate gene expression in cases of obesity and gastrointestinal tract inflammation.

嗜粘杆菌及其胞外囊泡对体外模型内源性大麻素系统的影响
最近的研究表明,肠道微生物群控制着宿主的免疫系统。益生菌使用不同的信号通路来调节肠道通透性、屏障完整性和能量平衡。方法:本研究通过RT-PCR和ELISA检测嗜黏液阿克曼氏菌及其胞外囊泡(EVs)对STC-1细胞系炎症及内源性大麻素系统相关基因的影响。结果:本研究结果表明,与感染的多重性(multiplicity of infections, MOI)相比,EVs对GLP-1表达有显著影响。值得注意的是,PYY和GLP-1基因的表达在所有处理中都有显著增加(p < 0.05)。相反,嗜粘单胞菌MOI为50、EV浓度为100 μg/mL时,STC-1细胞株中CB-1、CB-2和FAAH基因的表达显著降低(p < 0.05)。嗜粘单胞杆菌的MOI 50和100 μg/mL的EV浓度均能显著增强TLR-2基因的表达。相反,100 μg/mL浓度的ev显著降低了TLR-4基因的表达。在MOI为100和EV浓度为100 μg/mL时,A. muciniphila衍生的EV显著降低炎症因子(TNF-α和IL-6)水平,增加IL-10表达。这些结果提示嗜粘单胞杆菌及其ev可调节特定基因的表达,作为维持宿主能量平衡的靶点。结论:总之,本研究表明,嗜粘杆菌衍生的ev具有抗炎特性,并有可能调节肥胖和胃肠道炎症病例的基因表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Digestion
Digestion 医学-胃肠肝病学
CiteScore
7.90
自引率
0.00%
发文量
39
审稿时长
6-12 weeks
期刊介绍: ''Digestion'' concentrates on clinical research reports: in addition to editorials and reviews, the journal features sections on Stomach/Esophagus, Bowel, Neuro-Gastroenterology, Liver/Bile, Pancreas, Metabolism/Nutrition and Gastrointestinal Oncology. Papers cover physiology in humans, metabolic studies and clinical work on the etiology, diagnosis, and therapy of human diseases. It is thus especially cut out for gastroenterologists employed in hospitals and outpatient units. Moreover, the journal''s coverage of studies on the metabolism and effects of therapeutic drugs carries considerable value for clinicians and investigators beyond the immediate field of gastroenterology.
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